miR-637抑制结肠癌HCT116细胞生长和迁移的机制研究

Mechanism research of inhibiting the growth and migration of colon cancer HCT116 cells by miR-637

目的:分析miR-637对结肠癌HCT116细胞生长和迁移的影响。方法:分别构建miR-637过表达或者低表达的结肠癌HCT116细胞系。通过MTT方法检测miR-637对细胞增殖的作用,PI(propidium iodide)染色检测miR-637对细胞周期的影响,Annexin V-FITC/PI染色检测miR-637对细胞凋亡的作用。Western blot检测miR-637对细胞中CDK4和Bcl-2的影响。Transwell实验检测miR-637对细胞迁移的作用,进一步通过Western blot检测miR-637对细胞中MMP2的影响。结果:miR-637抑制HCT116细胞的增殖,抑制G_1/S期进程,促进细胞凋亡,Western blot结果显示,miR-637抑制CDK4和Bcl-2在HCT116细胞中的表达。Transwell实验指出,miR-637抑制细胞的迁移,Western blot结果指出miR-637抑制MMP2在HCT116细胞中的表达。结论:miR-637可以通过抑制CDK4、Bcl-2和MMP2的表达抑制HCT116细胞的生长和迁移。

Objective:To analyze the effect of miR-637 on the growth and migration of HCT116 cells.Methods:The HCT116 cell lines with overexpressed or low expression of miR-637 were constructed respectively.MTT was used to detect the effect of miR-637 on cell proliferation.PI(propidium iodide) staining was used to detect the effect of miR-637 on cell cycle.Annexin V-FITC/PI staining was used to detect the effect of miR-637 on apoptosis.The effects of miR-637 on CDK4 and Bcl-2 in cells were detected by Western blot.The effect of miR-637 on cell migration was detected by Transwell,and the effect of miR-637 on the MMP2 in cells was detected by Western blot.Results:miR-637 can inhibit the proliferation of HCT116 cells,inhibit G1/S phase progression,and promote cell apoptosis.Western blot results showed that miR-637 inhibited CDK4 and Bcl-2 expression in HCT116 cells.Transwell experiments showed that miR-637 inhibited cell migration.Western blot results showed that miR-637 inhibited MMP2 expression in HCT116 cells.Conclusion:miR-637 inhibited the growth and migration of HCT116 cells by inhibiting the expression of CDK4,Bcl-2 and MMP2.