不同高危型HPV检测方案对宫颈癌患者的筛查价值比较

Comparison of Screening Value of Defferent High-risk HPV Testing Program on Cervical Cancer

目的比较不同高危型人乳头瘤病毒(HPV)检测方案对宫颈癌患者的筛查价值,探讨宫颈癌早期筛查最佳方法。方法以120例健康体检的妇女作为研究对象,采用实时荧光定量PCR(PCR)及2代杂交捕获实验(HC2-HPV-DNA),检测高危型人乳头瘤病毒的感染情况。结果 PCR及HC2-HPV-DNA两种方法高危型HPV病毒阳性检出率分别为15.83%(19/120)和10.83%(13/120),两者比较有统计学意义。两种方法检测结果均为41~50岁及51~60岁年龄组中高危型HPV感染率明显较高。结论对于高危型HPV的检测HC2-HPV-DNA优于定量PCR,HC2-HPVDNA更适合大规模宫颈癌的早期筛选。

Objective To compare the screening value of defferent high-risk HPV testing program on cervical cancer , and to explore the best early detection methods of cervical cancer .Methods 120 cases healthy women were selected as the study objects.Real-time quanlititative PCR ( PCR) and Hybrid Capture 2 assay high-risk human papillomavirus DNA ( HC2-HPV-DNA) were used to detect the high-risk HPV infection in women .Results The positive rates of high-risk HPV virus of PCR and HC2-HPV-DNA were 15.83%(19/120) and 10.83% (13/120),the differece of the two methods was statistically significant . The results of the two methods showed that high-risk HPV infection in the patients 41~50 years old and 51~60 age group was significantly higher .Conclusion HC2-HPV-DNA is better than PCR ,and it is more suitable for large-scale early screening for cervical cancer .