IL13Rα2 SNPs在非小细胞肺癌中的表达及其临床意义

The Polymorphism of IL13Rα2 SNPs in Non-small Cell Lung Cancer and Its Clinical Significance

目的检测非小细胞肺癌(NSCLC)患者中IL13Rα2 SNPs(rs17095919)的多态性,并探讨其临床意义。方法收集NSCLC患者96例,另选取同期健康体检者40例作为正常对照。SNPs多态性测定应用Real-Time PCR Taqman分析。结果 NSCLC患者C/C、C/T和T/T基因型频率分别为22.9%、51.1%和26.0%,对照组分别为32.5%、30.0%和37.5%,C/C、T/T基因型表达两组间比较无显著性差异(P>0.05),而C/T基因型表达两组间比较具有统计学差异(P<0.05)。C/T基因型表达与NSCLC患者有无吸烟、肿瘤分化程度无显著相关(P>0.05),而与有无淋巴结转移、不同临床病理分期显著相关(P<0.05)。结论 IL13RA2 SNPs(rs17095919)C/T基因型与NSCLC的易感性密切相关,在NSCLC的早期诊断以及预后评估等方面起一定的作用。

Objective To investigate the polymorphism of IL13Rα2 SNPs( rs17095919) in non-small cell lung cancer( NSCLC) and its clinical significance. Methods 96 patients with NSCLC were enrolled in our study. Another 40 healthy person were usded as a normal control. The genotypes of IL13Rα2 SNPs( rs17095919) was performed using the Real-Time PCR Taq Man assays. Results The C / C,C / T,and T / T genotypes of IL13Rα2 SNPs( rs17095919) in NSCLC were 22. 9%,51. 1%,and26. 0%,respectively,and those of the control group were 32. 5%,30. 0%,and 37. 5%,respectively. There had no significant difference in C / C and T / T genotypes between the 2 groups( P > 0. 05). However,there was significant difference in C / T genotype between the 2 groups( P < 0. 05). The C / T genotype was not significantly related with smoking and histological grade( P > 0. 05).However,it was significantly related with lymphatic metastasis,and clinical pathological stage in NSCLC patients( P < 0. 05).Conclusion IL13RA2 SNPs( rs17095919) C / T genotype may be a susceptibility genotype in NSCLC. The detection of C / T genotype might be used as a mark for diagnosis,estimating the degree of differentiation or prognosis in NSCLC patients.