摘要
建立HPLC测定马甲子1#含量的方法,采用BDS HYPERSIL C18色谱柱(4.6 mm×100 mm×2.4μm),以乙腈-0.1%甲酸溶液为流动相,梯度洗脱,流速为1.0 m L/min,检测波长为320 nm。结果表明马甲子1#在0.021 4~0.854 4μg(r=0.999 9)范围进样量与峰面积线性关系良好,平均加样回收率为99.4%(RSD=1.18%)。该方法快速,准确,重现性好,可用于测定马甲子中马甲子1#的含量。
A high performance liquid chromatography(HPLC) was established to determine the paliurus ramosissimus 1#contents in Paliurus ramosissimus leaves. A BDS HYPERSIL C18 column(4.6 mm ×100 mm ×2.4 μm) with acetonitrile-0.1% formic acid solution as a mobile phase was adopted for gradient elution at the flow rate of 1.0 m L/min. The detection wavelength was 320 nm.The results showed that the linear relations of the sample size and the peak area was good and the average recovery rate was 99.4%(RSD=1.18%) when the paliurus ramosissimus 1# was between0.021 4 μg and 0.854 4 μg(r=0.999 9). This method is fast and accurate with high repeatability and can be used to measure the paliurus ramosissimus 1#content.
出处
《中国测试》
北大核心
2015年第4期43-45,共3页
China Measurement & Test
