摘要
用高效液相色谱法同时测定冬虫夏草中鸟嘌呤、胸腺嘧啶、腺嘌呤、腺苷、虫草素5种核苷类成分的含量。采用色谱柱为Aichrom Bond-AQ C18柱(250 mm×4.6 mm×5μm),以乙腈-水(7∶93)为流动相,流量为0.8 m L/min,检测波长254 nm,柱温30℃。鸟嘌呤、胸腺嘧啶、腺嘌呤、腺苷、虫草素的质量浓度分别在1.01~5.50μg/m L(r=0.9995)、0.05~1.01μg/m L(r=0.999 8)、0.20~1.98μg/m L(r=0.999 5)、1.01~5.07μg/m L(r=0.999 5)、0.10~1.03μg/m L(r=0.999 6)的范围内与峰面积线性关系良好,平均加样回收率分别为99.1%,97.8%,98.6%,100.4%,99.2%,RSD(n=5)分别为1.15%,2.34%,2.01%,1.75%,1.50%。该方法简便快速、准确度高、重现性好,可用于同时测定冬虫夏草中鸟嘌呤、胸腺嘧啶、腺嘌呤、腺苷、虫草素5种核苷类成分。
In order to simultaneously determine the accurate content of guanine,thymine,adenine,adenosine and cordycepin in Cordyceps sinenis by RP-HPLC method. An Aichrom Bond-AQ C18column(250 mm×4.6 mm× 5 μm) is used as the analytical column,and acetonitrile-water(7∶93)as the mobile phase,the flow rate is set as 0.8 m L/min,the detection wavelength is 254 nm and the column temperature is 30 ℃. The linear ranges of guanine,thymine,adenine,adenosine and cordycepin are 1.01~5.50 μg/m L(r=0.999 5),0.05~1.01 μg/m L(r =0.999 8),0.20~1.98 μg/m L(r =0.999 5),1.01~5.07 μg/m L(r=0.999 5),0.10~1.03 μg/m L(r=0.999 6),respectively. The mean recoveries of the five components are 99.1%,97.8%,98.6%,100.4%,99.2%,respectively. The RSD(n =5) is 1.15%, 2.34%, 2.01%, 1.75%, 1.50%, respectively. The method is simple,quick, accurate, reproducible, and can be used to determine the content of guanine, thymine,adenine,adenosine and cordycepin in Cordyceps sinenis at the same time.
出处
《中国测试》
北大核心
2015年第9期56-59,共4页
China Measurement & Test
