考来替兰磷酸盐结合率测定方法研究-离子色谱法

Determination of phosphate binding ratio of colestilan by IC

考来替兰磷酸盐结合率是临床前药物研究的一项重要内容,该文采用离子色谱法建立考来替兰对磷酸盐结合率的测定方法。采用电导检测器TSKguardcolumn Super IC-Anion HS保护柱及TSKgel Super IC-Anion HS色谱柱,淋洗液为Na_2CO_3(1.1mmol/L)/NaHCO_3(7.5mmol/L),流量为1.0 m L/min。结果表明,方法检出限为0. 02μg/m L,进样量在1.5～30μg/m L范围内线性关系良好(r^2=0.9994),精密度、重复性良好,测定结果的相对标准偏差为0.51%(n=6)。该方法简单方便、稳定可靠,可用于磷结合剂药物对磷酸盐结合率的测定。

The phosphate binding ratio of colestilan is an important part of preclinical drug research.The anions were detected using a suppressed conductivity detector,and TSKguardcolumn Super IC-Anion HS and TSKgel Super IC-Anion HS columns.The eluent used was a Na2 CO3(1.1 mmol/L)/NaHCO3(7.5 mmol/L)mixture and the flow rate was 1.0 m L/min.The detection limit of the method was 0.02μg/m L.The linear range was 1.5-30μg/m L(r2=0.9994).The RSD of determination results was 0.51%(n=6).This method is accurate,reliable and with good reproducibility,and this suggests preferred use of ion chromatography for the determination of phosphate binding ratio of phosphate binders.