工艺处理过程中病毒的灭活效果研究

Study on the Inactivated Effect and Significance of Virus in the Process of Inactivation

目的探究工艺处理对不同病毒的灭活效果及清除能力,为清除动物源性产品中病毒污染提供有效的灭活方法。方法选用新城疫病毒,人轮状病毒,伪狂犬病毒,猪细小病毒四种病毒作为指示病毒,加入所选材料中,经草酸和盐酸的清除工艺后,计算病毒初始及处理后的病毒滴度,总结工艺灭活效果。结果经过10%草酸1.5 h处理后,病毒滴度明显下降,但并未完全灭活;继续经过10%盐酸处理1h后,病毒滴度下降到零,病毒完全灭活。结论该酸处理工艺可以有效地清除病毒,达到良好的灭活效果,保证产品的安全性。

Objective To investigate the process of inactivation effect and scavenging ability of different virus,provide effective method for inactivating virus as the virus infection in animal products processing. Method laboratory select Newcastle disease virus,Human reoviruslike agent,Pseudorabies virus,Porcine Parvovirus as indicator virus,add to the selected material. In the removal process of oxalic acid and hydrochloric acid,lesion hole in the 96 hole plate,calculate the initial and after treatment virus titer of virus,summarize the process of inactivation. Result After treated with 10% oxalic acid 1. 5h,the virus titer decreased significantly,but not completely inactivated; continue treat with 10% hydrochloric acid 1h,the virus titer decreased to zero,virus completely inactivated. Conclusion acid treatment process can effectively remove the virus after inactivated treatment,achieve good disinfection effect,to guarantee the safety of the product and offer reference method of virus clearance of animal origin.