摘要
目的为谷氨酰胺(Glutamine,Gln)在正常鸭及鸭瘟(Duck plague,DP)模型中对肠道功能及作用途径的研究提供依据。方法 280只无特定病原体(Specific pathogen free,SPF)7日龄雏鸭随机分为8组,四组梯度剂量每天灌喂Gln(0、0.5、1、2 g/kg饲料)6d,及另四组灌喂同等梯度剂量Gln 6 d,于第一天灌喂30 min后攻0.2 m L2000TCID50鸭瘟病毒,观察Gln对正常雏鸭的免疫促进作用及攻毒鸭免疫应激的缓解作用。测定Toll样受体4(Toll-like receptor 4,TLR4)通路基因表达量。结果 Gln显著提高正常组TLR4通路mRNA表达量(P<0.05),显著降低攻毒组表达量(P<0.05)。结论 Gln通过TLR4通路提高正常雏鸭肠道的免疫力,降低鸭瘟病毒(Duck plague virus,DPV)造成的肠道免疫损伤。
Objective To investigate the effect of Glutamine( Gln) on the intestine physiological activity and the pathway in normal ducks and the models infected with the plague virus. Method 280 specific pathogen free( SPF)ducklings were randomly divided into eight groups. Gln was administrated at different doses( 0,0. 5,1,2 g / kg /feed / d) by oral gavage to the control groups and the model groups infected with 0. 2 m L duck plague virus( DPV)( 0. 2 m L 2000TCID50),respectively. The procedures were performed for 6 days. Effects of Gln in control and model ducklings were detected,and expression of the Toll like receptor 4( TLR4) pathway genes were determined.Result Expression of TLR4 pathway mRNAs was increased significantly in normal group( P < 0. 05),but reduced significantly in the model groups( P < 0. 05). Conclusion Gln improves immunity in duckling intestine through the TLR4 pathway,and resists the immune-mediated injury caused by DPV.
出处
《实验动物科学》
2015年第3期10-12,43,共4页
Laboratory Animal Science
基金
黑龙江省科技计划项目(No:PC11S01)
哈尔滨市科技平台建设项目(No:2014QA3BN002)
