一种比格犬失血性休克模型的建立

A Model of Hemorrhagic Shock in Beagle Dogs

目的将失血性休克模型程度统一到血气指标上,建立一种稳定的重度失血性休克大动物模型,以满足药效评价的需要。方法普通级比格犬15只,从颈动脉导管放血至动脉收缩压为50 mmHg左右并维持血压,模型成功标准为动脉血乳酸9～11 mmol/L、碱剩余<-12 mmol/L,静脉血氧饱和度<60%。模型成功后,观察实验动物一般生理指标、血气、血常规等指标变化。结果存活动物12只,模型成功率80%,具有较高的成功率和重现性,各指标变化符合失血性休克特征。结论该模型客观准确地模拟了失血性休克患者的临床特点,适用于血液制品及血液代用品的药效评价。

Objective To establish a model of hemorrhagic shock in Beagle dogs for the experimental study of drug evaluation and research.Method Fifteen Beagle dogs of common grade were bled until the arterial pressure(SBP)reached 50 mmHg,which was then maintained as best as possible.The criteria of the model were 9~11 mmol/L of Lac and 12 mmol/L of BE in arterial blood,less than 60%PO2 in venous blood.When the model was established,the changes of general physiological indexes,blood-gas parameters and blood routine indexes were observed.Result Twelve animals survived,the success rate of the model was 80%.The changes of indexes were in accordance with the characteristics of hemorrhagic shock.Conclusion The model was able to simulate the clinical characteristics of hemorrhagic shock objectively and accurately,and is suitable for the efficacy evaluation of blood products and blood substitutes.