HPLC法测定银翘解毒颗粒中绿原酸的含量

Determination of Chlorogenic Acid in Yinqiaojiedu Granules by HPLC

目的 :应用高效液相色谱法 (HPLC)测定银翘解毒颗粒中绿原酸的含量。方法 :采用C18柱 ,流动相为 0 .2mol·L-1磷酸二氢钠溶液 甲醇 (75∶2 5 ,pH 3.2 ) ,流速 1.0ml·min-1检测波长 32 7nm。结果 :绿原酸在 0 .2 4～ 1.2 μg范围内呈良好线性关系 (r =0 .9999) ;平均加样回收率为 99.3% ,RSD为 2 .0 %。结论 :本法操作简便 ,易行、重现性好 ,可用于银翘解毒颗粒的质量控制。

Objective: To establish a HPLC method for the determination of chlorogenic acid in Yinqiaojiedu Granules. Method: The column was C 18, the mobile phase was MeOH- 0.2 mol·L -1 N 2PO 4 (75∶25, pH 3.2), flow rate was 1.0 ml·min -1, The detecting wavelength was at 327 nm. Result: The linearity was good within a range of 0.24～ 1.2 μg (r= 0.999 9). The average recovery was 99.3%, RSD= 2.0%.Conclusion: This method is rapid, simple and accurate, and can be used for the quality control of Yinqiaojiedu granules.