HPLC法测定更昔洛韦氯化钠注射液中更昔洛韦的含量

Contend Determination in and Sodium Chloride Injection by HPLC

目的 :建立更昔洛韦氯化钠注射液中更昔洛韦的含量测定方法。方法 :采用BondapakC184 .6mm× 2 5 0mm ;流动相 :甲醇∶水 (10∶90 ) ,流速 :0 .8ml·min-1,检测波长 2 5 2nm。结果 :更昔洛韦加样回收率为 10 0 .1% ,线性范围 8～ 10 0ug·mL-1,RSD为 0 .2 % (n =9)。结论 :方法简便、灵敏、准确。

Objective: To establish a method to detect the contend of ganciclovir in sodium chloride injection.Method: Bondapak C 18 4.6×250 mm was used, fluid phase was methanol-water(10∶90),velocity was 0.8 ml·min -1,a detecting wavelength was at 252 nm. Result: recovery of ganciclovir was 100.1%,line arity was within a range of 8-100 μg·mL -1,RSD was 0.2%(n=9).Conclusion: This method is simple,sensitive, and accurate.