STAT6基因+2964G/A多态性与哮喘及血浆IL-5水平相关性研究

A study on the correlation between STAT6 gene+2964 G/A polymorphism and asthma and plasm IL-5 level.

目的探讨 STAT6基因+2964C/T 多态性与哮喘的相关性及对血浆 IL-5水平的影响。方法用聚合酶链反应和限制性片段长度多态性(PCR/RFLP)方法检测哮喘与对照组+2964位点多态性,用酶联免疫吸附法(ELISA)测定血浆 IL-5水平。结果 +2964位点等位基因 G、A 频率在两组间分布的差异有显著性(x^2=17.26,P<0.01),等位基因 G 与哮喘关联,OR(G/A)=2.62,95% CI=1.66～4.13.P<0.01。两组基因型(GG、GA、AA)频率的分布差异亦有显著意义(x^2=19.52,P<0.01),其优势比 OR(GG/AA)=3.30,95%Cl=1.26～8.66,P<0.05;OR(GG/GA)=3.79,95%,CI=2.03～7.09,P<0.01;OR(GA/AA)=0.87,95%CI=0.30～2.50,P>0.05,同组内,G 等位基因携带者血浆 IL-5水平高于非携带者;同一基因型,哮喘组 IL-5水平高于对照组。结论 STAT6基因+2964位点多态性是影响哮喘的重要候选基因,G 等位基因与哮喘关联,并能增加血浆 IL-5水平。

Objective To investigate the correlation between+2964 G/A locus polymorphism of IL-5 gene and the impact of the polymorphism upon plasm IL-5 level.Methods polymerase chain reaction/restriction fragment length polymorphism(PCR/RFLP)techniquers were used to determine+2964 locus polymorphism in asthma and control group,and plasm IL-5level were determined by ELISA.Results The frequency of allele G,A of+2964 locus had significant difference between two groups(x^2=17.26,P<0.01),allele G was associated with asthma, OR(G/A)=2.62,95%CI=1.66～4.13,P<0.01.The genetype(GG,GA and AA)distribution was signifi- cantly different between two groups(x^2=19.52,P<0.01),OR(GG/AA)=3.30,95% CI=1.26～8.66,P <0.05; OR(GG/GA)=3.79,95% CI=2.03～7.09,P<0.01; OR(GA/AA)=0.87,95% CI=0.30～ 2.50,P>0.05,In each group,the plasm IL-5 level of subjects with G allele was higher than that of subjects without G allele,the plasm IL-5 level of asthma group was higher than that of control group with same genetype.Conclusion The+2964 polymorphism of STAT6 gene may be an important candidate gene for asthma.G allele is associated with asthma and increases the plasm IL-5 level.