肝细胞肝癌中miR-19a与PTEN基因表达及意义临床研究

Expression level of miR-19a and PTEN in hepatocellular carcinoma tissues

目的通过检测miR-19a与其靶基因PTEN在肝癌组织中表达情况,探讨其在肝癌发病中的作用。方法采用荧光定量PCR技术检测miR-19a和PTEN基因在肝细胞肝癌和癌旁正常组织中转录表达水平。结果基因表达相对量采用癌症组/非癌组=2-△△CT进行数据分析,肝细胞癌组织中基因表达水平与癌旁正常组织相比,miR-19a表达增加,PTEN基因表达下降,差异均有统计学意义(P<0.05);肝癌组织中二者之间表达水平呈负相关(P<0.05)。结论 miR-19a在肝细胞肝癌中作为癌基因发挥作用,可以负性调节PTEN基因的表达。

Objective To explore the role of miR-19 and PTEN in the pathogenesis of hepatocellular carcinoma by detecting mir-19 a and PTEN expression in hepatocellular carcinoma tissues. Methods The transcriptional expression levels of mir-19 a and PTENgenes in hepatocellular carcinoma and the normal tissue adjacent to carcinoma were detected by fluorescence quantitative PCR.Results The expression level of mir-19 a in hepatocellular carcinoma was increased and PTEN was decreased compared with thatof the normal tissue adjacent to carcinoma, and the difference was statistically significant(P<0.05).There was a negative correlationbetween the expression levels in hepatocellular carcinoma.(P<0.05). Conclusion Mir-19 a plays an important role in hepatocellularcarcinoma as an oncogene, and can negatively regulate the expression of PTEN gene.