溃疡性结肠炎患者尿液NGAL、MMP-9检测的临床意义

Clinical significance of urine NGAL and MMP-9 in the patients of ulcerative colitis

目的观察活动期溃疡性结肠炎患者与健康志愿者尿液中性粒细胞明胶酶相关载脂蛋白(NGAL)和基质金属蛋白酶(MMP-9)水平的差异;观察溃疡性结肠炎活动期、缓解期尿液NGAL、MMP-9水平的差异。探讨溃疡性结肠炎患者尿液NGAL、MMP-9检测的可行性和临床意义。方法收集2011年6月—2016年6月在浙江省立同德医院诊治的溃疡性结肠炎患者,采用双抗体夹心酶联免疫吸附试验(ELISA)分别检测患者尿NGAL、MMP-9水平,观察活动期和缓解期患者尿NGAL、MMP-9水平的变化,并和健康对照组比较。结果活动期溃疡性结肠炎患者尿液NGAL水平明显高于健康对照组[(37.71±12.69)ng/mL vs.(19.27±5.34)ng/mL],差异有统计学意义(P<0.01)。活动期溃疡性结肠炎患者尿液MMP-9水平明显高于健康对照组[(5.14±1.25)ng/mL vs.(1.06±0.32)ng/mL],差异有统计学意义(P<0.01)。活动期溃疡性结肠炎经过治疗缓解的患者,治疗后尿液NGAL水平明显低于治疗前[(36.34±11.83)ng/mL vs.(25.13±5.76)ng/mL],差异有统计学意义(P<0.01)。活动期溃疡性结肠炎经过治疗缓解的患者,治疗后尿液MMP-9水平明显低于治疗前[(5.38±1.62)ng/mL vs.(3.09±0.67)ng/mL],差异有统计学意义(P<0.01)。结论活动期溃疡性结肠炎患者尿液NGAL、MMP-9水平明显高于健康对照组;经过治疗获得缓解的溃疡性结肠炎患者,活动期尿液NGAL、MMP-9水平明显高于缓解期。尿液NGAL、MMP-9检测方便、无创,患者无痛苦,可以作为监测溃疡性结肠炎病情的方法之一。

Objective To observe the difference of urinary levels of neutrophil gelatinase-associated apolipoprotein(NGAL)and matrix metalloproteinase-9(MMP-9)in patients with ulcerative colitis and healthy volunteers.To explore the clinical significance of detection of NGAL and MMP-9 in urine of patients with ulcerative colitis.Methods The levels of NGAL and MMP-9 in urine of patients with ulcerative colitis were detected by double antibody sandwich enzyme linked immunosorbent assay(ELISA).The changes of urinary NGAL and MMP-9 levels in active and remission patients were observed and compared with those in healthy controls.Results The levels of urinary NGAL in the patients of active ulcerative colitis were significantly higher than those in healthy controls((37.71±12.69)ng/mL vs.(19.27±5.34)ng/mL,P<0.01)The difference was statistically significant.The levels of urinary MMP-9 in the patients of active ulcerative colitis were significantly higher than those in healthy controls((5.14±1.25)ng/mL vs.(1.06±0.32)ng/mL,P<0.01)The difference was statistically significant.After treatments,the levels of urinary NGAL in patients with ulcerative colitis was significantly lower than those in active phase((36.34±11.83)ng/mL vs.(25.13±5.76)ng/mL,P<0.05)The difference was statistically significant.After treatments,the levels of urinary MMP-9 in patients with ulcerative colitis was significantly lower than those in active phase((5.38±1.62)ng/mL vs.(3.09±0.67)ng/mL,P<0.05)The difference was statistically significant.Conclusion The levels of urinary NGAL and MMP-9 in patients with active ulcerative colitis are significantly higher than those of control group.After treatments,the levels of urinary NGAL and MMP-9 in patients with ulcerative colitis are significantly lower than those in active phase.The detection of urinary NGAL and MMP-9 is convenient,non-invasive and no pain to the patient.It can be used as one of the monitoring methods of the ulcerative colitis.