一种快速鉴别三七粉中掺入伪品的鉴别方法

A quick method to identify the adulteration of Radix notogingseng powder

目的建立联合使用薄层色谱法和HPLC法鉴别三七粉中掺伪品的方法。方法采用薄层色谱法鉴别三七粉中掺入的三七茎叶、水田七与菊三七,展开剂为二氯甲烷-甲醇(体积比为40∶1);采用HPLC法建立三七药材的指纹图谱,并鉴别三七粉中掺入的三七茎叶皂苷提取物和水田七,采用Ultimate Plus C18(250 mm×4. 6 mm,5μm)色谱柱,流动相为乙腈-水,梯度洗脱,流速为1. 0 mL·min-1,柱温为25℃,检测波长为203 nm。结果薄层色谱法可鉴别出三七粉中掺入的三七茎叶和菊三七,最小可检出的掺入质量比分别为2%和20%; HPLC指纹图谱法可鉴别出三七粉中掺入的三七茎叶皂苷提取物和水田七,最小可检出的掺入质量比分别为2%和10%。结论本方法可作为三七粉的质量控制方法之一,以鉴别出掺入三七粉中的多种伪品。

Objective To establish a method for the identification of adulteration of Radix notogingseng powders by TLC and HPLC. Methods TLC method was used to identify the leaves of Panax notogingseng( Burk.) F. H. Chen and Gynura japonica Juel.,the developing solvent was dichloromethane-methanol( 40∶ 1). HPLC method was used to construct fingerprint,and identify the saponin extract of Panax notogingseng( Burk.) F. H. Chen and Schizocapsa plantaginea Hance;the Ultimate Plus C18( 250 mm × 4. 6 mm,5 microns) chromatographic column was used,mobile phase was acetonitrile-water,gradient elution and velocity for 1. 0 mL·min-1,column temperature was 25 ℃,detection wavelength was set at 203 nm. Results The TLC method could identify the leaves of Panax notogingseng( Burk.) F. H. Chen and G ynura japonica Juel. in the Radix notoginseng powder,and the minimum detectable amount was 2% and 20% respectively.HPLC fingerprint method could be used to identify the saponin extracts of Panax notogingseng( Burk.)F. H. Chen and Schizocapsa plantaginea Hance,and the minimum detectable amount was 2% and 10%respectively. Conclusion The methods can be used as control methods for Radix notoginseng powder to identify the various kinds of adulteration that mixed with Radix notoginseng powder.