HPLC-MS/MS法测定桑菊感冒丸中5种成分的含量

Content determinations of five components in Sangju Ganmao Pills by HPLC-MS/MS

目的建立高效液相色谱-串联质谱法同时测定桑菊感冒丸中芦丁、绿原酸、连翘苷、桔梗皂苷D和苦杏仁苷的含量。方法采用Waters Atlantis C18色谱柱(150 mm×2. 1 mm,5μm);以乙腈(A)-体积分数0. 1%甲酸(B)为流动相,梯度洗脱;流速0. 2 m L·min^(-1);柱温35℃;采用电喷雾离子源(ESI),以多反应监测(MRM)方式进行正离子扫描。结果芦丁、绿原酸、连翘苷和桔梗苷D分别在质量浓度0. 01～2 mg·L^(-1)内线性关系良好(r> 0. 999),苦杏仁苷在质量浓度0. 25～50 mg·L^(-1)内线性关系良好(r> 0. 999),平均回收率(n=6)分别为98. 82%、98. 96%、99. 43%、99. 19%和98. 88%,RSD分别为0. 7%、1. 3%、1. 4%、0. 8%和1. 1%。结论所建立的方法为桑菊感冒丸的全面质量控制提供科学依据。

Objective To establish the method for determinations of rutin,chlorogenic acid,forsythin,platycodin D and amygdalin in Sangju Ganmao Pills by HPLC-M S/M S. Methods The determination was carried out with Waters Atlantis C18column( 150 mm × 2. 1 mm,5 μm). The mobile phase consisted of acetonitrile( A)-0. 1% formic acid solution( B) with gradient elution at a flow rate of 0. 2 mL·min-1at35 ℃. The analytes were detected by tandem mass spectrometry with the electrospray ionization( ESI)source combined with multiple reaction monitoring( M RM) mode. Results Rutin,chlorogenic acid,forsythin and platycodin D showed a good linearity in the range of 0. 01-2 mg·L-1( r > 0. 999). The amygdalin showed a good linearity in the range of 0. 25-50 mg·L-1( r > 0. 999). The average recoveries( n = 6)were 98. 82%,98. 96%,99. 43%,99. 19%,98. 88%,with the RSD were 0. 7%,1. 3%,1. 4%,0. 8%,1. 1%. Conclusion This method is reliable,accurate,which offers advantages of high sensitivity and precision. It can be used for the overall assessment of the quality of Sangju Ganmao Pills.