二咖啡酰基奎宁酸对脑缺血大鼠转录组学的研究

Transcriptomic research of dicaffeoylquinic acid on cerebral ischemia rats

目的研究二咖啡酰基奎宁酸(MQA)对脑缺血大鼠RNA表达的影响作用。方法将24只雄性大鼠随机分为正常组、模型组与MQA组。各组大鼠术前连续灌胃6 d,再采用线栓法制备大鼠左侧脑缺血模型,术后24h将造模成功的大鼠进行解剖,取脑。将脑组织切块后进行RNA测序,检测模型组、正常组与MQA组的基因表达情况,再将数据进行基因表达丰度分析、GO富集分析及KEGG通路富集分析。结果模型组与MQA组之间存在762个差异基因,其中包括显著差异基因124个,极显著差异基因51个。通过GO富集分析发现,生物学过程相关基因整体呈现上调趋势,细胞组分相关的基因既有显著上调趋势也有显著下调趋势,分子功能相关的基因整体上呈现上调趋势,但也存在显著下调基因的项目;通过KEGG通路分析发现甲状腺激素信号通路相关基因呈显著下调,核糖体通路相关基因呈现部分上调及部分下调趋势。结论 MQA抗脑缺血可能跟改善纤溶系统、提高细胞骨架的稳定性、下调甲状腺激素信号通路有很大的关系。

Objective To study the effect of dicaffeoylquinic acid(MQA)on RNA expression in cerebral ischemic rats.Methods Twenty-four male rats were randomly divided into 3 group:normal group,model group and MQA group.Rats in each group were intragastrically administrated for 6 days before operation.The left cerebral ischemic model was established by the method of suture.The rats that had been successfully modeled were dissected 24 hours after surgery and the brains were removed.RNA sequencing was performed after the brain tissue was cut and the gene expression patterns of the model group,the normal group and the MQA group were detected.Then the data were analyzed for gene expression abundance analysis,Gene Ontology(GO)analysis and KEGG pathway analysis.Results There were 762 differentially expressed genes between the model group and the MQA group,including 124 genes with significant differences and 51 genes with most significant differences.After GO analysis,it was found that the genes related to biological processes showed an upward trend,and the genes associated with cellular components had both significant up-regulation and down-regulation,and genes related to molecular functions showed upward trends overall,but there were also significant down-regulated genes.Through KEGG pathway analysis,the genes related to thyroid hormone signaling pathway were significantly down-regulated,and the genes involved in ribosomal pathway were partially up-regulated and partially down-regulated.Conclusion The preventive effect of MQA on cerebral ischemia may be related to the improvement of fibrinolysis system,the improvement of cytoskeleton stability,and the down-regulation of thyroid hormone signaling pathway.