摘要
目的:探讨硫辛酸(lipoic acid LA)对实验性自身免疫性脑脊髓炎(experimental autoimmune encephalomy-elitis,EAE)大鼠的免疫抑制作用及其机制。方法:制备EAE动物模型,随机分为EAE组、LA治疗组和佐剂组。LA治疗组从免疫后(post-inoculation,免疫后)0d^5d每日腹腔内注射LA(100mg/kg)一次。在免疫后6d、8d、10d、12d、14d、16d处死动物,取脑和脊髓用免疫组化技术检测不同部位细胞间粘附分子-1(intercellular adhesion molecule-1,ICAM-1)和肿瘤坏死因子-a(tumor necrosis factor-a TNF-a)的表达。结果:LA治疗组临床症状评分(0.66±0.89)较EAE组(2.35±1.78)降低(P<0.05);LA治疗组体重减轻(7.63±3.47)较EAE组(12.60±3.53)减少(P<0.05);LA治疗组发病率(26%)较EAE组(67%)降低(P<0.05)。在免疫后10d^16d,LA治疗组ICAM-1的表达较EAE组降低(P<0.05),LA治疗组TNF-a的表达较EAE组降低(P<0.05)。结论:LA能够有效抑制EAE,其抑制机制与LA下调ICAM-1、TNF-a的表达有关。
Objective To study the immunosuppressive effect of LA and its mechanism in rats with experimental autoimmune encephalomyelitis(EAE).Methods Wistar rats were randomly divided into EAE group,LA group and Complete Freund Adjuvant(CFA) group.LA group were injected with LA(100 mg/kg)once a day from 0 to 5 p.i..The brain and spinal cords were removed on days from 6 to 16p.i.for immunohistochemical staining of ICAM-1 and TNF-a in different parts of nervous system.The correlation between ICAM-1 and TNF-a in EAE group was analyzed.Results When compared with EAE group(2.35±1.78),the clinical symptom of LA group(0.66±0.89)was statistically significantly decreased(P<0.05).The weight loss of LA group(7.63±3.47) which was compared with EAE group(12.60±3.53) decreased obviously(P<0.05).The incidence of LA group(26%) which was compared with EAE group(67%)were decreased obviously(P<0.05).On days 10 to 16 p.i.,the expression of ICAM-1 in LA group were degraded(P<0.05),when compared with those of EAE group,the expression of TNF-a in LA group were degraded(P<0.05),when compared with those of EAE group.Conclusion LA is a potential agent to inhibit EAE effectively.The inhibiting mechanism of LA is related to down-regulation of ICAM-1 and TNF-a.
出处
《实用医技杂志》
2008年第6期682-685,共4页
Journal of Practical Medical Techniques
基金
人事部06年度回国人员科技活动择优资助项目(项目编号:国人厅发[2006]164号
项目负责人:马存根)
山西省2003年度归国留学人员科研基金资助项目(项目编号:2003-69
项目负责人:马存根)
