摘要
[目的]通过Northern印迹杂交(Northern blot)验证人参中微小核糖核酸(miRNA)的存在。[方法]根据已知人参miRNA序列设计合成2条特异LNA反义寡核苷酸探针,并用地高辛标记。采用通用植物miRNA提取试剂盒提取新鲜人参Total RNA,15%变性PAGE电泳分离小RNA并检测。[结果]Northern blot表明,人参miR482和miR1439等2种miRNA均有表达。[结论]验证了人参中miRNA的存在,为研究人参的疗效机制、临床应用和剂型开发等提供新思路。
[Objiective]To verify the micro Ribonucleic Acid(miRNA)in Panax ginseng C.A.Mey.(PG).[Methods]Based on 2 published miRNA sequences of PG,2 DIG-labeled antisense probes were synthesized.The total RNAs from PG were extracted with Plant Micro RNA Extraction Kit,followed the steps provided by the kit,and than loaded onto 15% denaturing polyacrylamide gel and hybridized with the appropriate DIG-labeled probes.[Results]Northern blotting detected miR482 and miR1439 in PG.[Conclusion]The result presents the evidence of miRNA in PG and may lead to novel approaches to the research of PG.
出处
《实用中医内科杂志》
2012年第8S期7-8,10,共3页
Journal of Practical Traditional Chinese Internal Medicine
基金
广东省自然科学基金博士启动项目(7300243)
