针灸联合中药对肾小球细膜细胞增殖影响随机平行对照研究

The Randomized Controlled Study on Acupuncture Combined with Traditional Chinese Medicine Treatment Effect on the Proliferation of Glomerular Mesangial Cell

[目的]观察针药结合治疗对大鼠正常肾小球细膜细胞增殖影响。[方法]60只SD大鼠购进后适应性喂养一周,于第二周第一天将BSA 1mg溶于0.5mL的0.01MPBS溶液中,加0.5mL弗氏不完全佐剂,混匀成悬浊液,给大鼠做多点皮下注射。皮下注射一周后,以BSA 2.5mg溶于1mLPBS溶液中尾静脉注射,4次/周,共3周。静脉注射3周后,收集24h的尿液进行尿蛋白的检测。根据24h的尿蛋白量,使用随机平行对照方法,将大鼠编号按随机数字表方法分为六组。分别为针药结合组(选取穴位:肾俞、脾俞、命门、伏兔、三阴交、太溪、足三里、关元。针刺方法:以平补平泻为主一日针腹部伏兔、三阴交、太溪、足三里、关元穴位,留针五至十分钟。隔日,用艾灸背部穴位肾俞、脾俞、命门5min,如此循环;灌胃壮肾方25g(原药材)/kg/d,针灸组(除不给壮肾方外,其余治疗与针药结合组一致),中药组(壮肾方原药材25g/kg),阳性药组(肾炎康复片0.6mg/kg),模型组(等体积生理盐水),空白对照组(等体积生理盐水),每组10只,治疗8周。动脉取血,分离血清备用。用MTT法检测不同给药组大鼠血清对正常大鼠肾小球细膜细胞增殖的影响。[结果]针药结合组能显著抑制体外培养肾小球细膜细胞的增殖,与模型组比较有显著性差异(P<0.01)。[结论]针药结合治疗可通过抑制GMC的过度增殖活化,而减轻肾小球系膜增生。

[Objective]observe acupuncture combined therapy on normal rat glomerular mesangial cell proliferation fine.[Methods]60 SD rats were fed for one week after the purchase,in the second week of the first day of the BSA 1mg 0.01MPBS solution dissolved in 0.5mL,0.5mL plus incomplete Freund's adjuvant,and mix into the overhang turbid liquid,rats do more subcutaneous injection.Week after subcutaneous injection to BSA 2.5mg dissolved 1mLPBS solution intravenously,4 times / week for 3 weeks.Intravenous injection of 3 weeks after the urine collected 24h urinary protein detection.According 24h urine protein,the use of randomized controlled method,the rats were randomly number method is divided into six groups.Namely acupuncture combined group(selected points:Shenshu,Pishu,Mingmen,Futu,Sanyinjiao,Taixi,Zusanli,Guan Yuan.Acupuncture Methods:December reinforcing-reducing needle-based Futu,Sanyinjiao,Taixi,Zusanli,Guan Yuan,the needle five to ten minutes.every other day,with moxibustion acupuncture back Shenshu,Pishu,Mingmen 5min,so the cycle;gavage Zhuang kidney side 25g(raw herbs)/ kg / d,the acupuncture group(except the parties themselves do not give strong kidney,and the remaining group treated with acupuncture combined with consistent),medicines(Zhuang Shen Fang original medicinal 25g/kg),positive group(nephritis rehabilitation tablets 0.6mg/kg),model group(equal volume of saline),control group(equal volume of saline),n = 10,8 weeks of treatment.arterial blood serum was separated spare.different administration by MTT assay in serum of normal glomerular mesangial cell proliferation fine.[Results]acupuncture combined could significantly inhibit the in vitro proliferation of glomerular thin film,compared with the model group were significantly different(P <0.01).[Conclusion]acupuncture combined therapy by inhibiting activation of the GMC excessive proliferation and reduce glomerular mesangial proliferation.