开心散对快速老化痴呆小鼠SAMP8炎症因子及β-APP影响随机平行对照研究

Kai Xin San to the Rapid Aging Dementia Model SAMP8 Mice Inflammation Factor and the Influence of the Beta APP

[目的]观察开心散对快速老化痴呆小鼠SAMP8炎症因子及β-APP影响。[方法]使用随机平行对照方法,将40只SAMP8小鼠按随机数字标法分为4组,10只/组,模型对照组、开心散组(高、低剂量组)、艾地苯醌组。正常对照组10只SAMR1小鼠。采用ELISA双抗体夹心法测定干预后脑组织及血清TNF-α、IL-8和β-APP含量。[结果]与正常对照组比较,模型组小鼠脑组织TNF-α、IL-8和β-APP含量明显升高(P<0.01),血清TNF-α、IL-8和β-APP浓度明显降低(P<0.01);与模型组比较,开心散低剂量组、开心散高剂量组小鼠脑组织TNF-α、IL-8和β-APP含量明显降低(P<0.01),血清TNF-α、IL-8和β-APP浓度明显升高(P<0.01)。[结论]开心散可减少脑组织的β-APP含量,降低炎症因子TNF-α、IL-8水平。

[Objective]Effect of kaxin-san on inflammatory factor andβ-APP of senescence accelerated mouse/prone 8(SAMP8). Methods:By random parallel control method,40 SAMP8s were randomly divided into the model group,high-does kaxin-san group,low-does kaxin-san group and idebenone group,10 rats in each group. 10 SAMR1s were adopted as normal control group. The levels of TNF-α,IL-8and β-APP in brain tissue and serum of SAMP8 by double antibody enzyme linked immunosorbent assay(ELISA).Results:Compared with control group,the contents of TNF-α,IL-8 and β-APP in brain tissue of model group was increased(P<0.01),the concentration of TNF-α,IL-8 and β-APP in serum of model group was decreased(P<0.01);Compared with model group,the contents of TNF-α,IL-8 and β-APP in brain tissue of high-does kaxin-san group,low-does kaxin-san group was decreased(P<0.01),the concentration of TNF-α,IL-8 andβ-APP in serum of high-does kaxin-san group,low-does kaxin-san group was increased(P<0.01). Conclusion:kaxin-san can exert neuroprotective effects by decrease the contents of TNF-α,IL-8 and β-APP in brain tissue.This may be important mechanisms of kaxin-san in improving the abilities of learning and memory.