藤龙补中汤对大肠癌RKO细胞癌药靶基因表达的影响

Effects of Teng-Long-Bu-Zhong-Tang on expression of cancer drug target genes in colorectal carcinoma RKO cells

目的 观察藤龙补中汤对人大肠癌RKO细胞癌药靶基因表达影响。方法 藤龙补中汤作用RKO细胞,TRIzol试剂提取RNA,试剂盒纯化RNA,药靶基因PCR芯片(Cancer Drug Targets PCR Array)检测基因表达。结果 藤龙补中汤作用RKO细胞后,PLK3和TP53表达升高,ABCC1、AURKA、BCL2、BIRC5、CDK2、CDK4、FLT4、HDAC1、HIF1A、MDM4、MTOR、NFKB1和PLK1表达降低,涉及细胞增殖、细胞周期、凋亡、细胞衰老、多药耐药、转移、淋巴管生成和表观遗传修饰。结论 藤龙补中汤可调控RKO细胞多个癌药靶基因表达,与藤龙补中汤对大肠癌细胞增殖、细胞周期、凋亡、细胞衰老、转移和表观遗传作用相关;藤龙补中汤还可能对大肠癌多药耐药和淋巴管生成有抑制作用。

Objective To observe the effects of Teng-Long-Bu-Zhong-Tang(TLBZT)on expression of cancer drug target genes in human colorectal carcinoma RKO cells.Methods RKO cells were treated with TLBZT.RNA was extracted by TRIzol regent and purified with commercial kit.Genes expression were detected by Cancer Drug Targets PCR array.Results After TLBZT treatment,PLK3 and TP53 were up-regulated,ABCC1,AURKA,BCL2,BIRC5,CDK2,CDK4,FLT4,HDAC1,HIF1 A,MDM4,MTOR,NFKB1 and PLK1 were down-regulated in RKO cells.These genes involve cell proliferation,cell cycle,apoptosis,cell senescence,multidrug resistance,metastasis,lymphoangiogenesis and epigenetic modification.Conclusion TLBZT regulates expression of multiple genes of cancer drug targets and are related to the effects of TLBZT on cell proliferation,cell cycle,apoptosis,cell senescence,metastasis and epigenetics in colorectal cancer.TLBZT may also inhibit lymphoangiogenesis and multidrug resistance in colorectal cancer.