白介素-1受体拮抗剂对致敏豚鼠的抗喘作用(英文)

Anti-asthmatic effects of interleukin-1 receptor antagonist in sensitized guinea pigs

目的 :观察白介素 1受体拮抗剂 (IL 1ra)对致敏豚鼠的抗喘作用。方法 :用卵白蛋白致敏豚鼠 ,用张力换能器和MedLab软件记录气道平滑肌的肌张力和收缩活动。结果 :整体实验时 ,在 0 .3 3～9.0mg·kg- 1 剂量范围内 ,IL 1ra能使组胺 乙酰胆碱及卵白蛋白诱发的致敏豚鼠哮喘的潜伏期延长 ,动物跌倒率降低。此作用具有剂量依赖性。其中 ,9.0mg·kg- 1 剂量组的作用与异丙基肾上腺素相同 ;离体实验时 ,3× 1 0 - 7mol·L- 1 以上浓度的IL 1ra对气管平滑肌具有直接松弛作用 ,其EC50 为 5 .88×1 0 - 7mol·L- 1 ;1 0 - 7mol·L- 1 的IL 1ra能对抗卵白蛋白引起的气管平滑肌肌张力的增加 ;3×1 0 - 7mol·L- 1 以上剂量组的IL 1ra预防给药则能使卵白蛋白诱发的气管平滑肌的收缩程度显著降低 ,其IC50 为 4.48× 1 0 - 7mol·L- 1 。结论 :IL 1ra能直接松弛致敏豚鼠的气管平滑肌 ,拮抗多种抗原诱发的致敏豚鼠的哮喘发作。

AIM: To observe the anti asthmatic effects of interleukin 1 receptor antagonist (IL 1ra) in sensitized guinea pigs. METHODS: Sensitized guinea pigs were made by ovalbumin, and the tension and contraction of tracheal smooth muscles were measured by force displacement transducer and MedLab software. RESULTS: In the doses of 0.33 - 9.0 mg·kg -1 , IL 1ra prolonged the asthmatic incubation period and reduced animal tumble ratio in asthma guinea pigs induced by histamine acetylcholine or ovalbumin. These effects were dose dependent. In vitro, IL 1ra could directly relax tracheal smooth muscles up the dose of 3×10 -7 mol·L -1 and the EC 50 value was 5.88 ×10 -7 mol·L -1 . IL 1ra antagonized the increase of trachea tension stimulated by ovalbumin at the dose of 10 -7 mol·L -1 . Moreover, IL-1ra prevented tracheal smooth muscle contraction induced by ovalbumin up the dose of 3×10 -7 mol·L -1 , and the IC 50 value was 4.48 ×10 -7 mol·L -1 . CONCLUSIONS: IL 1ra can directly relax tracheal smooth muscles in sensitized guinea pigs, and antagonize asthma caused by many kinds of antigens.