黄芪总提物对体外脂多糖刺激的枯否细胞基质金属蛋白酶-9活性的影响

Effects of total extraction of Astragalus on activity of matrix metalloproteinase-9 from Kupffer cells in rat liver

目的 :研究黄芪总提物 (totalextractionofAs tragalus,TEA)对体外脂多糖 (LPS)刺激的枯否细胞(KC)基质金属蛋白酶 9(MMP 9)活性的影响。方法 :采用IV型胶原酶灌流分离大鼠肝枯否细胞进行原代培养 ,用LPS刺激枯否细胞并收集上清液 ,酶谱法观察TEA对肝枯否细胞产生MMP 9的影响。结果 :TEA (5～ 40mg·L- 1 )对LPS(1 μg·ml- 1 )刺激的大鼠肝KC细胞MMP 9活性有明显的抑制作用。结论 :TEA对体外激活的肝枯否细胞产生MMP 9有明显的抑制作用 。

AIM: To study the effect of total extraction of Astragalus (TEA) on activity of matrix metalloproteinase 9 (MMP 9) from Kupffer cells in rat liver. METHODS: The Kupffer cells (KC) were isolated from livers of normal rats by in situ perfusion with pronase and collagenase and density gradient centrifugation with refrigerated centrifuger. Then KC were cultured with LPS ( 0.1 μg·ml -1 ) and MMP 9 was measured with zymography. RESULTS: TEA (5～40 mg·L -1 ) could inhibit the activity of MMP 9 induced by LPS. CONCLUSION: TEA has inhibitory efficacy on activity of matrix metalloproteinase 9 (MMP 9) from Kupffer cells in rat liver.