虫草菌丝对实验性肝硬化大鼠肝组织胰岛素酶活性的影响

Dynamical influence of Cordyceps sinensis on activity of hepatic insulin ase of experimental liver cirrhosis in rats

目的 :探讨虫草菌丝对实验性肝硬化大鼠肝组织匀浆上清液胰岛素酶活性的动态影响及其可能机制。方法 :以CCl4诱导大鼠肝硬化模型 ,设立正常组、模型组和虫草组。正常组于实验开始时便处死大鼠 ,而后 2组则于造模 3 ,6,9wk分批处死 ,取血及肝组织标本。生化法测定血清丙氨酸转氨酶(ALT)、清蛋白 (ALB)含量 ,放射免疫法测定血清透明质酸 (HA)、血清胰岛素 (INS)含量及肝组织匀浆上清液的胰岛素酶活性。结果 :(1 ) 3 ,6wk虫草组大鼠血清ALT ,ALB及HA含量与对应模型组差异无显著意义 (P >0 .0 5 ) ,9wk虫草组大鼠血清ALT ,HA含量显著低于对应模型组 (P <0 .0 5 ) ,而其血清ALB含量则显著高于对应模型组 (P<0 .0 5 ) ;(2 ) 3wk虫草组及模型组大鼠血清INS水平及肝组织胰岛素酶活性与正常组大鼠差异无显著意义 (P >0 .0 5 ) ,6,9wk虫草组及模型组大鼠血清INS水平显著高于正常组 (P <0 .0 5 ) ,而对应的肝组织胰岛素酶活性则显著低于正常组 (P <0 .0 5 ,或P <0 .0 1 ) ;(3 ) 3 ,6,9wk虫草组大鼠血清INS水平及肝组织胰岛素酶活性与对应模型组差异无显著意义(P >0 .0 5 )。结论 :虫草菌丝可减轻CCl4对肝细胞的损伤 ,并可抑制肝纤维化 ;造模 6wk后实验性肝硬化大鼠肝组织胰岛素酶活性开始降低 。

AIM: To investigate the dynamical effects and mechanisms of Cordyceps sinensis(CS) on the activity of the hepatic insulinase of experimental liver cirrhosis in rats. METHODS: The liver cirrhosis models were induced by CCl 4 rats were randomly allocated i nto a normal group, a model group and a CS group.Rats in normal group were sacr ificed at the beginning of experiment. While other two groups were sacrificed r andomly at the end of wk 3, wk 6 and wk 9. Blood and tissue specimen were taken. Biochemical assay was used to determine the level change of ALT, ALB content in serum. And radioimmunological assay was used to determine the level change of HA, INS con tent in serum and the activity of the hepatic insulinase. RESULTS: (1) There wer e no significant differences of the serum level of ALT, ALB, HA between the CS g roup and the model group at wk 3 and wk 6 (P >0.05 ). The serum level of ALT, HA in the CS group was lower than that in the model group at wk 9 (P <0.05 ),but the serum level of ALB in the CS group was higher than that in the model group at wk 9 (P <0.05 ).(2) There were no significant differe nces of the serum level of the insulin and the activity of the hepatic insulinas e between the CS group and normal group at wk 3 (P >0.05 ). The serum level of insulin in the CS group and model group at wk 6 and the wk 9 were higher than that in the normal group (P <0.05 ).But the according activity of the hepatic insulinase was lower than that in the normal group (P <0.05 ,or P <0.01 ).(3)There were no significant differences of the s erum level of the insulin and the activity of the hepatic insulinase between the CS group and the model group at wk 3, wk 6 and the wk 9 (P >0.05 ). CONCLUSION: Cordyceps sinensis may decrease the destroy of the hepatocyte by CCl 4, and inhibi t the hepatic fibrogenesis. After 6 wk CCl 4 administration,the activi ty of the hepatic insulinase begin to decrease and the level of serum insulin be gin to decrease and the level of insulin begin to increase. Cordyceps sinensi s can't inhibit the decreasing of the activity of the hepatic insulinase, so t hat it can not improve the hyperinsulinism either.