HPLC法测定驳骨丸中阿魏酸的含量

Determination of Ferulic Acid in Bogu Pill by HPLC

目的 建立驳骨丸中阿魏酸的含量测定方法。方法 在室温条件下超声波提取阿魏酸,采用HPLC法测定其含量,ODS色谱柱,以甲醇-乙腈-1％冰醋酸(15:15:70)为流动相,检测波长323nm。结果 阿魏酸在0.56μg～11.20μg/mL范围内线性关系良好(r=0.9996),平均回收率为100.26％,RSD=1.48％。结论 该方法简便、灵敏、准确,可用于驳骨丸的质量控制。

Objective To establish a method for the content determination of ferulic acid in Bogu Pill(BP). Methods Ferulic acid was obtained by ultrasonic extraction at room temperature and detected by HPLC. HPLC was performed with ODS chromatographic column, methanol-acetonitrile-1%acetic acid(15: 15: 70) as mobile phase and detection wavelength at 323 nm. Results The linearity of ferulic acid was in the range of 0.56 μg～11.20 μg/mL(r=0.9996), and the average recovery was 100.26%, RSD=1.48%. Conclusion This method was efficient and can be used for the quality control of BP.