大鼠脊髓损伤后神经生长因子对内皮素含量的影响

Effects of Nerve Growth Factor on Endothelin after Spinal Cord Injury in Rats

目的 探讨神经生长因子对大鼠脊髓损伤保护作用的机理。方法 采用改良Allen’s法 10g× 2 5cm致伤大鼠第 12胸椎脊髓 ,经蛛网膜下腔导管分别于术后即刻、1、4、8、2 4、72h各注入神经生长因子溶液 ,并与生理盐水组和正常对照组作对照。采用放射免疫方法测定内皮素含量。结果 与正常组相比较内皮素含量在伤后均显著升高 (P <0 0 1) ,神经生长因子治疗组与生理盐水组相比较 ,在伤后 4、8、2 4、72h ,内皮素含量明显下降 (P <0 0 1)。结论 神经生长因子通过抑制脊髓损伤后继发作用 ,抑制了内皮素导致的恶性循环 。

Objective To investigate the protective mechanisms of nerve growth factor (NGF) on spinal cord injury. Methods The spinal cord injury (SCI) of Wistar rats was performed by a 10 g× 2 5 cm impact on the posterior T 12 spinal cord. The experimental animals received NGF liquid by subarachnoids space tube. The radioimmuological techniques are applied to examine the level of endothelin. Results The level of endothelin was significantly increased after the injury as compared with that in control group ( P <0.01). The level of endothelin in NGF group were obviously lowered as compared with that in normal saline group 4 h after injury ( P <0.01). Conclusion NGF can protect spinal cord against injury in vivo. One of mechanisms is that NGF prohibits the damage circulation of endothelin.\;