小檗碱联用环孢素诱导免疫耐受后对肾功能的影响

The effect of recipient kidney function by Ber coadministration CsA used to induce immune tolerance in rats of allogenic cardiac transplantation

目的 研究小檗碱联用环孢素诱导同种异基因移植免疫耐受后对受体肾功能的影响。方法 采用Ono’s方法建立同种异基因大鼠心脏异位移植免疫耐受模型 ,将实验动物分为 5组。对照组 (Wistar Wistar) :每天注射生理盐水共2 1d ;安慰剂组 (SD Wistar) :每天注射生理盐水共 2 1d ;CsA组 (SD Wistar) :每天按 2mg·kg-1剂量注射环孢素A(Cy closporineA ,CsA)共 2 1d ;Ber组 (SD Wistar)每天按 16mg·kg-1剂量灌服小檗碱 (Berberine,Ber)共 2 1d ;CsA +Ber组(SD Wistar) :每天按 2mg·kg-1剂量注射CsA ,16mg·kg-1剂量灌服Ber共 2 1d ,观察受体血清尿素氮 (BUN)、肌酐(Cr)指标水平的变化。结果 联用Ber后 ,降低了受体长时间使用CsA后的尿素氮 (BUN)和肌酐 (Cr)水平。

AIM To investigate the effect of recipient kidney function by CsA coadministration Ber used to induce immune tolerance in rats of allogenic cardiac transplantation. METHODS The authors established the SD to Wistar rats heterotopic cardiac transplantation model by Onos methods.Observe the cardiac allograft survival and levels of BUN and Cr in the recipients plasma. The recipients were classified into 5 groups randomly after heterotopic cardiac transplantation were performed. Group A (Wistar to Wistar)): Received placebo intraperitoneal injected for 21 days; Group B (SD to Wistar): Saline intraperitoneal injected for 21 days; Group C (SD to Wistar):CsA 2 mg·kg -1 ·d -1 intraperitoneal injected for 21 days; Group D(SD to Wistar):Ber 16 mg·kg -1 ·d -1 gastrointubation for 21 days; Group E(SD to Wistar): Ber 16 mg·kg -1 ·d -1 gastrointubation coadministration CsA 2 mg·kg -1 ·d -1 ip for 21 days. RESULTS The levels of BUN and Cr in recipint plasma is lower evidently compare with the group with CsA ip simply. CONCLUSION Ber can reduce the renal toxicity in recipients by CsA which was intraperitoneal injected (ip) over a long period time.