灯盏花素对UL补硒大鼠谷胱甘肽过氧化物酶和硫氧还蛋白还原酶基因表达和活性的影响

Effects of Scutellarin on glutathione peroxidase and thioredoxin reductase gene expression and activities in rats supplemented with upper limit Selenium

目的 观察灯盏花素对允许的最高摄入量硒大鼠肝脏、肾脏、睾丸组织中谷胱甘肽过氧化物酶 (GSH Px)和硫氧还蛋白还原酶 (TR)基因表达和活性的影响。方法 采用RT PCR方法和酶活性测定分析GSH Px和TR基因表达水平和活性。结果 UL硒能降低大鼠肝脏组织中GSH Px和TRmRNA表达水平及活性 ,灯盏花素能增加补硒大鼠肝脏中GSH Px和TRmRNA表达水平和活性。UL硒对大鼠肾脏、睾丸组织GSH Px和TRmRNA表达水平和活性无明显影响。结论 灯盏花素能有效拮抗UL硒所致的GSH Px和TRmRNA表达和活性的下降 ,且UL硒所致的GSH Px和TRmRNA表达和活性的下降具有组织特异性。

AIM To study the effects of Scutellarin on glutathione peroxidase and thioredoxin reductase gene expression and activities in liver, kidney and testis tissues of rats supplemented with upper limit intake (UL) of Se. METHODS RT PCR and enzyme activities were used to determine GSH Px and TR mRNA levels and activities. RESULTS GSH Px and TR mRNA levels and activities were remarkably decreased in livers of rats supplemented with UL Se. Scutellarin significantly increased GSH Px and TR mRNA levels and activities in livers of rats supplemented with UL Se. No significant effects were found in rats treated with only Scutellarin as compared with the control. There were also no significant effects of UL Se on GSH Px and TR mRNA levels and activities in rat kidney and testis. CONCLUSION Scutellarin effectively antagonized the decrease of GSH Px and TR mRNA levels and activities in rat livers due to supplementation with UL. The effect of UL on GSH Px and TR mRNA levels shows tissue specificity.