期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

血红素蛋白质在甲基纤维素膜中的电化学研究 被引量：3

The Direct Electrochemistry of Heme -proteins Entrapped in Methyl Cellulose Hydrogel
下载PDF
导出
摘要 利用甲基纤维素(MC)水凝胶将肌红蛋白(Mb)、血红蛋白(Hb)、辣根过氧化物酶(HRP)和过氧化氢酶(Cat)四种血红素蛋白质固定在裂解石墨电极表面,形成稳定的血红素蛋白质-MC膜修饰电极.在MC膜中,Mb、Hb、HRP和Cat直接与电极之间传递电子.四种血红素蛋白质的式电势都随溶液pH的增加而负移且呈线性关系,表明电子传递过程伴随着质子转移.这种氧化还原玻尔效应反应了蛋白质结构与功能的关系. Four heme proteins, including myoglobin, hemoglobin, horseradish peoxidase and catalase, were immobilized on edge -plane pyrolytic graphite (EPG) electrodes by methyl cellulose. The proteins entrapped in the MC film undergo fast direct transfer - electron reactions. The Eo. are b'nearly dependent on solution pH, indicating the electron transfer of FeIII/FeII redox couple companied with the transfer of proton.
作者 李业梅
机构地区 郧阳师范高等专科学校化学系
出处 《郧阳师范高等专科学校学报》 2003年第3期33-36,38,共5页 Journal of Yunyang Teachers College
基金 校级2003年度科研项目[2003A05]
关键词 肌红蛋白 血红蛋白 辣根过氧化物酶 过氧化氢酶 甲基纤维素 电化学 myoglobin hemoglobin horseradish peroxidase catalase methyl cellulose electrochemistry
分类号 O646 [理学—物理化学]
  • 相关文献

参考文献9

  • 1Yamazaki I,Araiso T,Hayashi Y,et al.Analysis of Acid-Base Properties of Peroxidase and Myoglobin[].Advances in Biophysics.1978
  • 2Nassar AEF,Zhang Z,et al.Proton-Coupled Electron Transfer from Electrodes to Myoglobin in Ordered Bi-omembrane-like Films[].Journal of Physical Chemistry B.
  • 3Rusling J F.Enzyme Bioelectrochemistry in Cast Biomem-brane-like Films[].Accounts of Chemical Research.1998
  • 4Kundu P P,Kundu M.Effect of salts and surfactant and their doses on the gelation of extremely dilute solutions of methyl cellulose[].Polymer.2001
  • 5Armstrong F A,Wilson G S.Recent Development in Faradaic Bioelectrochemistry[].Electrochimica Acta.2000
  • 6Ondaz A A,Bedioui F.The Electrocatalytic Reduction of Organohalides by Myoglobin and Hemoglobin in a Bi-omembrane-like film and Its Application to the Electro-chemical Deterction of Pollutants: New Trends and Dis-cussion[].Sensors and Actuators B Chemical.1999
  • 7Gorton L,Lindgren A,Larsson T,et al.Direct Electron Transfer between Heme-containing enzymes and elec-trodes as basis for third generation biosensors[].Analytica Chimica Acta.1999
  • 8O’ Dea J J,Osteryoung J G.Characterization of Quasi-Reversible Surface Processes by Square-Wave Voltam-metry[].Analytical Biochemistry.1993
  • 9Bond A M.Modern Polarographic Methods in Analytical Chemistry[]..1980

同被引文献16

  • 1廖静敏,李光,马念章.碳纳米管在生物传感器中的应用[J].传感技术学报,2004,17(3):467-471. 被引量：20
  • 2李业梅,刘慧宏,张志凌,关成明.过氧化氢酶在琼脂糖膜中的电化学研究[J].分析科学学报,2004,20(6):569-572. 被引量：3
  • 3Lippard S J Berg J M 席振峰 姚光庆 项斯芬 等.生物无机化学原理[M].北京:北京大学出版社,2000..
  • 4[1]Iijima S.Helical microtubes of graphitic carbon[J].Nature,1991,354:56-58.
  • 5[3]Chen X,Xie H,Kong J,et al.Characterization for didodecyldimethylammonium bromide liquid crystal film entrapping catalase with enhanced direct electron transfer rate[J].Biosens Bioelectron.2001,16(1-2):115-20.
  • 6[4]Zhang Z,Salem C H,Richard S,et al.Direct Voltammetry and Enzyme Catalysis with M tuberculosis Catalase-Peroxidase,Peroxidases and Catalase in Lipid Films[J].Anal Chem,2002,74:163-170.
  • 7[8]Wyman J Q.Regulation in macromolecules,as illustrated by hemoglobin[J].Quart Rev Biophys,1969,1:35-42.
  • 8[9]Bond A M.Modern Polarographic Methids in Analytical Chemistry[M].New York:Marcel Dekker,1980:27-45.
  • 9[10]Yamazaki I,Araiso T,Hayashi Y,et al.Analysis of Acid-Base Properties of Peroxidase and Myoglobin[J].Adv Biophys,1978,11:249-281.
  • 10LippardSJ BergJM.席振峰 姚光庆 项斯芬等译.生物无机化学原理[M].北京:北京大学出版社,2000.2..

引证文献3

二级引证文献1

郧阳师范高等专科学校学报
2003年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部