单细胞电泳法检测肿瘤患者化疗后淋巴细胞DNA损伤

The Application of SCGE-KIAS in Monitoring of DNA Damage in Lymphocytes of Tumor Patients Treated with Cyclophosphamide

应用单细胞电泳 (SCGE)检测肿瘤患者环磷酰胺化疗后外周血T淋巴细胞DNA损伤。在标准SCGE条件下应用彗星图象分析系统定量分析人T淋巴细胞DNA的损伤程度。彗星图象分析研究结果显示 ,肿瘤患者外周血T淋巴细胞DNA损伤明显强于正常对照组 ,肿瘤患者外周血T淋巴细胞拖尾动量为 10 42± 1 98,正常人群为 1 2 6± 0 77;两者差异极显著 (P <0 .0 1)。肿瘤患者化疗后T淋巴细胞的拖尾长度为 3 3 69± 7 56μm ,DNA损伤的百分比为 3 1 5± 5 46% ;正常人群T淋巴细胞的拖尾长度和DNA损伤的百分比分别为 16 2± 1 5μm和7 46± 1 15% ;两组数据相比差异极显著 (P <0 .0 1)。结论 :单细胞电泳可快速灵敏地检测细胞DNA损伤 ,能用于肿瘤化疗患者的流行病学观察。

Single cell gel electrophoresis assay (SCGE), a lso named as alkaline comet assay, was a simple, rapid and sensitive method to evaluate DNA damage. In this study SCGE technique was used to monitor DNA damag e difference in tumor patients caused by chemotherapy, DNA damage distribution frequency and DNA damage characters were analyzed by kom et image analysis system (KIAS). The results showed that cyclophosphamide greatl y caused DNA damage in lymphocytes of tumor patients. There was significant diff erence of peripheral blood lymphocyte DNA damage between tumor patients and heal thy controls. Tail length of lymphocytes were 33.69±7.56 μm, and tail DNA% we re 31.51±5 4 6% in 10 cancer patients treated with cyclophosphamide, while Tail length were 1 6.2±1.5 μm and tail DNA% were 7.46±1.15% in healthy controls. there was great significant difference on tail length and tail DNA% values between cancer patie nts and healthy controls (P<0.01). In conclusion, the successful measurement of DNA damage caused by Cyclophosphamide treatment means that the alkaline come t assay as a valuable tool can be very useful in cancer epideminology study, and also be valuable to evaluate DNA damage status of patients in clinic.