鞘内新斯的明对福尔马林致痛大鼠的抗伤害作用机理的初步研究

Study on the Mechanism of Antinociception of Intrathecal Neostigmine in the Formalin Test in Rats

目的 探讨新斯的明 (Neo)对福尔马林致痛大鼠镇痛机理。方法 雄性 SD大鼠 4 0只 ,随机分为对照组和实验组 ,对照组又分为 NS组和 F组 ,实验组又细分为 Ne F组、L a Ne F组和 L n Ne F组。 NS组为正常对照组 ,F组给予 5 %福尔马林 10 0 μl,足底注射 ,Ne F组、L a Ne F组及 L n Ne F组在同 F组处理前分别给予 Neo、L-精氨酸(L- Arg) +Neo鞘内注射 (ith)、L- NG-硝基精氨酸甲酯 (L- NAME,NOS抑制剂 ) +Neo ith,在福尔马林处理后分别检测大鼠脊髓 Fos的表达及观察大鼠的行为学表现。结果 Ne F组的缩腿舔爪时间显著短于 F组 ,脊髓的 Fos表达显著弱于 F组 ,预先给予 L- Arg或 L- NAME分别能加强及抑制以上作用。结论 新斯的明能引起大鼠脊髓背角 NO的释放 ,抑制 Fos表达可能是其产生抗伤害作用的机制之一。

Objective To assess the effect of intrathecal neostigmine and its possible mechanism in formalin test. Methods Forty healthy male SD rats were randomly divided into control groups(NS, F)and test groups(NeF, LaNeF, LnNeF).Group NS served as normal control.Group F was treated with 5% formalin 100 μl, s.c. Group NeF, LaNeF, LnNeF were treated respectively with Neo 10 μg, L Arg 4.7 μmol ith,L NAME 370 nmol ith before receiving 5% formalin 100 μl, s.c like group F. 0 1 h and 2 h after formalin treatment, behavior responses were observed and spinal cord segments were removed for expression of Fos. Results The flinching and licking time of group NeF was shorter than that of group F, and the Fos expression of group NeF was weaker than that of group F. Pretreatment of L Arg or L NAME potentiated or reduced neostigmine induced responses respectively. Conclusion Intrathecal neostigmine could induce nitric oxide release in the spinal cord, the suppression of Fos expression might be one of the antinociception mechanisms of intrathecal neostigmine in the formalin test.