孕晚期小鼠脾脏巨噬细胞抗原提呈功能的改变

A Change in Antigen Presentation Ability of Spleen Macrophage from Balb/c Mouse during Late Pregnancy

目的 研究孕晚期 Balb/ c小鼠脾脏巨噬细胞抗原提呈功能的改变。方法 分离孕晚期小鼠脾脏巨噬细胞 ,以动情期小鼠作为对照。通过诱导迟发超敏反应和体外淋巴细胞抗原特异性转化实验检测巨噬细胞使机体初始 T致敏的能力以及诱导致敏 T细胞增殖的能力。结果 将充分摄取和结合了抗原 HGG或 Pla- Ag的孕晚期小鼠脾脏巨噬细胞注射到动情期小鼠腹腔使之致敏 ,再用相同抗原注射到小鼠足底诱发的迟发超敏反应强度显著低于动情期小鼠 (P<0 .0 5 )。当孕晚期小鼠脾脏巨噬细胞被当作抗原提呈细胞时 ,体外用人γ球蛋白抗原或胎盘抗原诱导致敏的正常小鼠的 T细胞增殖强度也显著低于动情期小鼠 (P<0 .0 5 )。孕晚期小鼠脾脏巨噬细胞还能显著抑制动情期小鼠脾脏巨噬细胞刺激致敏 T细胞增殖的能力。结论 孕晚期 Balb/

Objective To study the antigen presentation ability of spleen macrophage from Balb/c mouse during late gestation. Methods The spleen of Balb/c mouse during late gestation was collected aseptically and macrophage was separated; the mice in estrus were used as control. The ability of macrophage to prime original T cells to human gamma globulin antigen (HGG Ag) or placenta antigen (Pla Ag) was evaluated by a delayed type hyper sensitivity (DTH) response induced by the same antigen, and the ability of macrophage to induce the proliferation of primed normal T cells to HGG Ag or Pla Ag was assessed by an antigen special lymphocyte transformation in vitro. Results After being sensitized previously by spleen macrophage from Balb/c mice during late gestation that has been pulsed by HGG Ag or Pla Ag, the DTH intensity of mice response to the same antigen was significantly weaker than that being sensitized by macrophage from mice in estrus( P <0.05). When spleen macrophage from Balb/c mice during late gestation was used as antigen presentation cell (APC), the proliferability of primed T cell induced by HGG Ag or Pla ag was significantly lower than that when macrophage from estrous Balb/c mouse was used in vitro ( P <0.05). Conclusion The antigen presentation ability of spleen macrophage from Balb/c mouse is inhibited during late pregnancy. This may be the important mechanism by which maternal immune system tolerates embryo antigen and may be responsible for the down regulation of maternal cell mediated immunity during pregnancy.