多药耐药相关蛋白1在骨肉瘤中的表达

Expression of Multidrug Resistance-associated Protein 1 in Osteosarcoma and Its Relationship with Clinicopathologic Characteristics

目的 探讨骨肉瘤组织中多药耐药相关蛋白 1(MRP1)的表达及其与临床病理特征的关系。方法 采用免疫组织化学方法检测 6例正常成年骨组织和 4 5例骨肉瘤组织中 MRP1,结合临床病理指标进行分析。结果6例正常骨组织中 MRP1的表达均为阴性。 4 5例骨肉瘤中 MRP1阳性 32例 (71.11% ) ,其中高分化骨肉瘤阳性率2 8.5 7% (2 / 7) ,中、低分化骨肉瘤阳性率 78.95 % (30 / 38) ,两组间差异有显著性 (P<0 .0 5 )。且随着骨肉瘤分化程度的降低 ,MRP1表达率升高 ,MRP1的表达与骨肉瘤的恶性程度呈正相关 (r=0 .84 4 )。根据肿瘤大小、Enneking外科分期、血清碱性磷酸酶 (AL P)含量、患者性别、年龄及就诊前病程长短等分组 ,MRP1表达无统计学意义。结论正常成年骨组织中 MRP1表达阴性。骨肉瘤组织中有 MRP1的表达 ,MRP1的表达与骨肉瘤的恶性程度呈正相关 ,提示 MRP1可能为骨肉瘤原发耐药的重要原因之一。

Objective To detect the expression of multidrug resistance associated protein 1(MRP1) in human ostesarcoma and to explore its relationship with clinicopathologic characteristics. Methods Six normal bone specimens and 45 osteosarcomas from patients were analysed for MRP1 expression by immunohistochemistry.The expression levels of MRP1 with the clinicopathologic parameters of the patients were examined using Chi square test. Results The expression of MRP1 was observed in 32 (71.11%) osteosarcoma specimens.More( P <0.05) specimens expressed MRP1 in high grade osteosarcomas(30/38,78.95%) than in low grade osteosarcomas(2/7,28.57%).The correlation coefficient between expression of MRP1 and grade of pathology was 0.844. In a limited number of patients, the expression of MRP1 was not related to the the age and sex of the patients, Enneking surgical stage, osteosarcoma size, serum concentration of ALP and duration before diagnosis.No expression of MRP1 in 6 normal bone specimens was observed. Conclusion MRP1 is expressed in osteosarcoma and its expression is positively correlated with the malignancy of osteosarcoma.