二尖瓣病变患者左房心肌总蛋白激酶C活性及亚类含量的变化

Study on the Total Activity of PKC and the Quantity of PKC(α,β) in Left Auricle Tissues of the Patients with Mitral Disease and Atrial Fibrillation

目的 探讨二尖瓣病变患者左房心肌总蛋白激酶 C(PKC)活性及 PKCα、β亚类含量与心房颤动(AF)之间可能存在的关系。方法 35例患者分为四组 ,A组 :二尖瓣狭窄 (MS)伴 AF;B组 :为窦性心率 (SR)的二尖瓣狭窄 ;C组 :二尖瓣关闭不全 (MR)伴 AF;D组 :为 SR的二尖瓣关闭不全。分别检测各组总 PKC活性及 PKCα、PKCβ亚类含量。结果 各组间总 PKC活性无明显差异 (P>0 .0 5 )。伴有 AF的二尖瓣病变患者 (MS或 MR)其左房心肌 PKCα含量低于 SR的二尖瓣病变患者 (MS或 MR) ;而 PKCβ含量却高于 SR的二尖瓣病变患者 ,但上述差异尚未达到统计处理的显著性水平 (P>0 .0 5 )。结论 左房心肌总 PKC活性与二尖瓣病变患者 AF发生的关系不明显 ,但左房心肌 PKC亚类含量水平的变化与

Objective To investigate the possible relation of atrial fibrillation (AF) with the total activity of protein kinase C (PKC) and the quantity of PKC (α,β) in the left auricle tissues of the patients with mitral disease. Methods Thirty five patients hospitalized for valve replacement surgery were divided into four groups: group A(simple mitral stenosis with AF), group B(simple mitral stenosis with sinus rhythm), group C(simple mitral regurgitation with AF), group D (simple mitral regurgitation with sinus rhythm), The total PKC, activity and the quantity of PKC α and PKC β were measured and compared in an attempt to find out whether significant difference exists between the groups. Results There was no significant difference in total PKC activity between the four groups ( P >0.05). The quantity of PKC α in patients with AF was lower than that in patients with sinus rhythm. the quantity of PKC β in patients with AF was higher than in patients with sinus rhythm. But these differences were not statistically significant ( P >0.05). Conclusion Under conditions of the same rhythm and the same herat disease, the total PKC activity and the quantity of PKC (α, β) of the four groups showed no significant difference. No obvious relation was seen between the total activity of PKC in left auricle tissue and the occurrence of AF in patients with mitral disease, but more research on the relation between AF and the quantity of PKC isoforms in left auricle tissues would be needed.