不同方法建立大鼠实验性牙周炎模型的比较研究

A Comparative Study Utilizing Different Methods for Establishing an Experimental Periodontitis Model in Rats

目的 比较牙颈部丝线结扎 +高糖水、牙颈部丝线结扎 +高糖水 +牙周致病菌引起牙周炎的病程是否相同。方法 4 0只 5周龄 SD大鼠 ,随机分为对照 (N)组、牙颈部丝线 +高糖 (P1 )组 (以 10 %的糖水代替饮水 )、牙颈部丝线结扎结扎 +牙龈卟啉单胞菌 +高糖 (P2 )组、牙颈部丝线结扎 +牙龈卟啉单胞菌 +具核梭杆菌 +高糖组(P3) ,并在不同时间段进行微生物学及组织学检查。结果 微生物学检查显示加入的细菌均定植成功 ,且加入的细菌为大鼠牙周的优势菌。组织学结果显示 P1 组在 2周时形成龈炎 ,4周时形成较典型的牙周炎 ;P2 组 2周时表现为急性期牙周炎 ,4周时表现为慢性牙周炎 ;P3组的病程与 P2 基本相似 ,但病变程度更为严重。结论 牙颈部丝线结扎

Objective To evaluate the experimental periodontitis in rats induced by ligature plus high sugar water with and without infection of periodontopathic bacteria. Methods Forty SD rats (5 weeks of age) were randomly divided into a control group (N) and three experimental groups (P 1, P 2 and P 3). All experimental groups were given silk ligatures around the cervix of the second maxillary molars and were provided with drinking water containing 10% glucose. Group P 2 was inoculated with P. gingivalis, three times; group P 3 was inoculated with P. gingivalis and F. nucleatum, three times. At weeks 0, 2 and 4, microbiological and histological examinations were made; alveolar bone loss was stereoscopically measured as the distance from the cement enalmel junction to the alveolar crest. Results Microbiological examination indicated that the bacteria inoculated in groups P 2 and P 3 were successfully colonized and both of P. gingivalis and F. nucleatum were predominant among the bacterial colonies of the examined sites. At week 2, histological examination showed gingival erosion and alveolar bone loss in all experimental groups and periodontal pockets in groups P 2 and P 3. The most serious lesions were observed in group P 3. At week 4, the periodontal destruction in all experimental groups became more severe. Conclusion Ligature, high sugar water combined with mixed infection of periodontopathic bacteria can successfully induce experimental periodontitis in rats.