原发性高血压病患者血管紧张素转换酶的基因多态性分析

The Polymorphism of Angiotensin in converting enzyme of essential hypertension patients

目的 采用三条引物法进行血管紧张素转换酶 (ACE)基因分型 ,并与Rigat法进行比较 ,探讨ACE基因多态性与原发性高血压病 (EH)之间的关系。方法 抽取外周血DNA ,分别用三条引物法和Rigat法对 12 8例EH患者进行ACE基因分型 ,并以 15 0例正常人作对照组。 结果 Rigat法对DD型的错判率为 2 2 .2 2 %。正常血压人群ACE基因DD型、Ⅱ型、ID型分别有 10、62、78例 ,占 6.67%、41.3 3 %、5 2 % ,I和D的等位基因频率是 67%、3 3 % ;原发性高血压病人群ACE基因DD型、Ⅱ型、ID型分别有 14、47、67例 ,占 10 .94%、3 6.72 %、5 2 .3 4% ,I和D的等位基因频率是 63 %、3 7%。

Objective Use the Triple Primer Method to explore the relationship between polymorphism of ACE gene and essential hypertension.At the same time,comparing with the different results of Rigat method and the Triple Primer Method.Methods 128 patients with the essential hypertension were genotyped by both Rigat method and the Triple Primer Method,150 normotensive subjects were genotyped by the Triple Primer Method only.Results When 18 DD individuals genotyped by Rigat method were retyped using the Triple Primer Method,4 of them(22%) were retyped as ID.Genotypes DD,ID and II of ACE gene accounted for 6.67% and 10.94%,52% and 52.34%,and 41.33% and 36.72%,respectively,in the normotensives and essentialhypertension.Frequencies of I and D alleles for ACE gene were 67% and 63%,and 33% and 37%,respectively,in the normotensives and essential hypertension.Conclusion The polymorphism of angiotensin in converting enzyme was implicated in essential hypertension population.