低浓度2,4-DCP对鲫鱼肝脏抗氧化防御系统的影响

Influence of low-level 2,4-DCP on the antioxidant defense system in livers of Carassius auratus.

采用室内模拟方法,以2,4-二氯苯酚(2,4-DCP)和小鲫鱼(Carassius auratus)为实验材料,研究了0.1mg/L 2,4-DCP在不同暴露期内对鲫鱼肝脏抗氧化防御系统的影响.结果表明,过氧化氢酶(CAT)、超氧化物歧化酶(SOD)及氧化型谷胱甘肽(GSSG)对2,4-DCP非常敏感,在暴露第2天就受到极显著的诱导或抑制(P<0.01);还原型谷胱甘肽(GSH)含量总体上被抑制;谷胱甘肽还原酶(GR)活性整体上呈现轻微诱导趋势谷胱甘肽过氧化物酶(Se-GPx)活性先受抑制后被诱导;谷胱甘肽硫转移酶(GST)的活性先被诱导,后降低到对照组水平.上述肝脏抗氧化酶指标可作为水生生态系统中2,4-DCP污染的生物监测指标.

Adopting indoor simulation technique with 2,4-Dichlorophenol (2,4-DCP) and Carassius auratus as experimental materials to study for the first time the influence of 0.1mg/L 2,4-DCP on the antioxidant defense system in the liver of the fish. Catalase (CAT), superoxide dismutase (SOD) and oxidized glutathione (GSSG) were very sensitive to 2,4-DCP; and induced or inhibited very significantly at the second day of exposure (P<0.01). The contents of reduced glutathione (GSH) were inhibited on the whole. The activities of glutathione reductase (GR) tended to be induced slightly on the whole. The activities of selenium-dependent glutathione peroxidase (Se-GPx) were inhibited first and then induced. The activities of glutathione S-transferase (GST) were induced first and then lowered to the control level. These hepatic antioxidant indices mentioned above could be considered as biological monitoring indices of 2, 4-DCP pollution in aquatic ecosystems.