肝郁脾虚因素对S180荷瘤小鼠肿瘤生长和T细胞免疫功能的影响

Influence of the Factor of Liver Depression and Spleen Deficiency on the Growth of Tumour and the Immune Function of T-cells in S180 Tumor-bearing Mice

目的 观察肝郁脾虚因素对S180荷瘤小鼠肿瘤生长和T细胞免疫功能的影响。方法 取腹腔传代第 7天的S180肿瘤细胞 ,调细胞浓度至 1× 10 10 L-1,接种于昆明小鼠右腋皮下 ,0 2mL 只。接种后当天用束缚加饮食失节的方法造成肝郁脾虚证 ,并设单纯肝郁脾虚证、单纯肿瘤和正常对照组。 10d后处死小鼠 ,剥取肿瘤称瘤重 ,取脾脏检测T淋巴细胞增殖及产生IL 2、IFN γ的能力 ,并摘取胸腺称重计算胸腺指数。结果 肝郁脾虚因素可明显增加S180荷瘤小鼠肿瘤重量 (P <0 0 1) ,降低荷瘤小鼠脾T淋巴细胞增殖和产生IL 2和IFN γ的能力 (P <0 0 1) ,并可使小鼠胸腺指数明显降低 (P <0 0 1)。结论 肝郁脾虚因素可加重抑制荷瘤小鼠的细胞免疫功能 ,对肿瘤生长有促进作用。

Objective To observe the influence of the factor of liver depression and spleen deficiency (LDSD) on the growth of tumour and the immune function of T cells in S180 tumor bearing mice. Methods Tumor cells were extracted from the abdominal cavity of S180 tumor bearing mice and adjusted to a concentration of 1×10 10 L -1 . The S180 tumor cells were inoculated into the Kunming mice by subcutaneous injection at the right oxter at a dose of 0 2 mL per mouse. LDSD syndrome was induced in the inoculated mice on the very day of the inoculation by the method of binding and an intemperate diet. At the same time, the single LDSD group, single S180 tumor bearing group and normal control group were also set up for the experiment. After the 10 th day of the inoculation, the mice were executed. The weight of tumor, the proliferation rate of T cells in the spleen, the ability to produce IL 2 and IFN γand the thymic index were measured. Results It was found that in the S180 tumor bearing mice, the LDSD factor could markedly increase the tumor weight ( P <0 01), decrease the proliferation rate of T cells ( P <0 01) and the ability to produce IL 2 and IFN γ( P <0 01), and lower the thymic index. Conclusion The LDSD factor can aggravate the suppression of cellular immune function and promote the growth of tumor in the tumor bearing mice.