腰痛舒胶囊对一氧化氮诱导的软骨细胞凋亡的影响

Effects of Yaotongshu Capsules on Apoptosis of Rabbit Chondrocytes Induced by Nitric Oxide

目的 观察腰痛舒胶囊对一氧化氮在体外诱导的兔软骨细胞凋亡的影响。方法 体外培养的兔软骨细胞 ,分别加入硝普钠 (SNP)培养 12、2 4、4 8h ,用透射电镜和流式细胞仪观察软骨细胞凋亡的变化 ,并以不同剂量腰痛舒胶囊含药血清加入NO诱导的软骨细胞凋亡体系培养 2 4h ,采用annexinV PI方法检测凋亡情况。结果 在体外培养兔软骨细胞加入硝普钠 2 4h ,流式细胞仪检测凋亡率为 (74 0 5± 6 6 8) % ,对照组为 (3 15± 0 76 ) % (P <0 0 1) ;透射电镜可见较典型凋亡细胞形态改变 ,加入不同剂量腰痛舒胶囊含药血清 ,其凋亡率明显下降 ,并以高剂量最为显著。结论 硝普钠在体外能诱导软骨细胞凋亡 ;高剂量腰痛舒含药血清能明显降低NO诱导的软骨细胞凋亡 ,提示腰痛舒胶囊能抑制软骨细胞凋亡。

Objective To observe the effects of Yaotongshu Capsules(YC)on apoptosis of rabbit chondrocytes induced by nitric oxide (NO) in vitro. Methods The transmission electron microscope (TEM) and the flow cytometer (FCM) were used to observe the apoptosis of the rabbit chondrocytes which were cultured with sodium nitroprusside (SNP) for 12, 24 and 48 hours, respectively; and the Annexin V/PI method was used to detect the status of apoptosis of chondrocytes induced by NO and again cultured for 24 hours in the presence of different doses of YC medicated serum. Results The FCM observation showed that the rate of apoptosis was 74 05±6 68% in the chondrocytes cultured with SNP for 24 hours, while that in the control group was 3 15±0 76% ( P <0 01). The TEM observation revealed a typical morphological change of apoptotic chondrocytes. The rate of apoptosis was remarkably lowered by different doses of YC medicated serum, especially by the large dose of YC medicated serum. Conclusion SNP can induce apoptosis of rabbit chondrocytes in vitro and the large dose of YC medicated serum can remarkably decrease the rate of apoptosis of chondrocytes induced by NO, which suggests that YC can inhibit apoptosis of rabbit chondrocytes.