911-FITC荧光探针与免疫细胞的结合实验

Binding experiments of 911-FITC with Immunocytes

应用911-FITC荧光探针对胸腺、脾脏淋巴细胞、巨噬细胞和内皮细胞进行了荧光染色,采用流式细胞仪及荧光显微镜对细胞的染色情况进行了评价。结果表明,911-FITC荧光探针能与淋巴细胞、巨噬细胞和内皮细胞发生特异性结合。证明911可通过与免疫细胞直接结合而起到免疫调节作用。

Lymphocytes from thymus, spleen, macrophages and endothelial cells w.ere incubated with the fluorescent probe of 911-FITC and the colouring method with flow cytometry and fluorescent microscope was reviewed in this paper. The results indicated that 911-FITC could specifically bind to lymphocytes, macrophages and endothelial cells. These findings suggested that 911 could play a immunomodulatory effect by direct combination with immu-nocytes.