乌贼墨诱导小鼠巨噬细胞产生白细胞介素-1的研究

Study on Secretion of Interleukin-1 Induced by Sepia in Mice

目的:应用乌贼墨,研究其诱导BALB/C鼠巨噬细胞产生白细胞介素-1(interleukin-1)。方法:给小鼠ig灌入乌贼墨,抽取腹腔液,应用成纤维细胞增殖实验检测诱导巨噬细胞产生IL-1的生物学活性,体内持续时间和不同稀释浓度促细胞增殖的结果。结果:乌贼墨能诱导BALB/C鼠巨噬细胞产生IL-1,且分泌活性较强,与对照组比较有显著性差异。体内跟踪检测可见停止诱生后第2天达高峰,第5天分泌活性仍明显,第9天接近正常。IL-1待检液稀释8倍时活性无明显变化,32倍时近正常水平。结论:乌贼墨能诱导小鼠巨噬细胞产生IL-1,48h分泌活性最高。

Objective To investigate secretion of interleukin-1 (IL-1) in BALB/C mice macro-phage induced by sepia. Methods Ascitic fluid was drawed out after sepia was poured into mice intragastrically and the biologic activity of IL-1 induced in mice macrophage was examined by fibroblast proliferation experiment. Duration of IL-1 secretion and the concentration- activity relation in vivo were also studied. Results Sepia can induce IL-1 in BALB/C mice macrophage. The effects had significant differences compared with control group. Tracking detection in vivo displayed IL-1 had the highest secretion at the second day after stopped inducing and also secretion evidence at the fifth day and reached to normal at the ninth day. The IL-1 activity of original-detected liquid had not decreased at 8 fold dilution and approach normal at 32 fold. Conclusion Sepia can induce IL-1 in BALB/C mice macrophage, and it had shown the highest secretion was at 48 hours.