HPLC测定乌苓苁蓉口服液中2,3,5,4’-四羟基二苯乙烯-2-O-β-D葡萄糖苷的含量

Determination of 2,3,5,4’-Tetrahydroxydiphenylethylene-2-O-β-D-glucoside in Wuling Congrong oral solution by HPLC

目的 建立乌苓苁蓉口服液中 2 ,3,5 ,4’ -四羟基二苯乙烯 - 2 -O - β -D葡萄糖苷的测定方法。 方法 采用KromasilC18色谱柱 (4.6mm× 2 0 0mm ,5 μm) ;流动相为甲醇 - 0 .0 1mol·L-1醋酸水溶液 (2 0∶80 ) ;检测波长 32 0nm。结果 线性范围 6 0 .1～ 6 0 1ng ,r=0 .9995 ,平均回收率为 10 2 .2 6 %,RSD =0 .71%。结论 所用方法简便 ,快速 ,可靠。

OBJECTIVE To describe an accurate,stable and practicable method for determining 2,3,5,4'-tetrahydroxydiphenylene-2-O-β-D-glucoside in Wuling Congrong oral solution by HPLC.METHODS The Kromasil C 18 column, and methanol-0.01 mol·L -1 acetic acid(20∶80) as the mobile phase were adopted and the detective wavelength was 320 nm. RESULT The calibration curves was linear in the range of 60.1-601 ng(r=0.9995).The average recovery was 102.26%,RSD=0.71%. CONCLUSION The method is simple,rapid and reliable.