HPLC测定心神宁片中栀子苷的含量

Determination of geniposide in Xinshen Ning tablets by HPLC

目的 建立心神宁片中栀子苷的含量测定方法。方法 采用KromasilC18不锈钢色谱柱 (2 0 0mm× 4.6mm) ,以乙腈 -水(15∶85 )为流动相 ,检测波长 2 38nm。结果 栀子苷在 0 .2 48～ 1.488μg范围内 ,线性关系良好 (r =0 .9999) ,平均回收率为99 .0 6 %,RSD =0 .99%(n =6 )。结论 所建立的方法专属性强 ,灵敏度高 ,重复性好 ,可作为心神宁片中栀子苷的测定方法。

OBJECTIVE To establish a determination method of geniposide in Xinshen Ning tablets.METHODS HPLC was performed,using acetonitrile-water (15∶85)as mobile phase and detective wavelength was 238 nm. RESULTS The linearity of this method was good, and average recovery was 99.06%, RSD=0.99% (n=6).CONCLUSION This method was simple,accurate,and available for the quality control of Xinshen Ning tablets.