端粒酶反义寡核苷酸对体外培养ACC-M细胞的影响

The Effect of Telomerase AS-ODN on Human ACC-M Cell Line

目的 研究端粒酶反义寡核苷酸 (AS ODN)对体外培养人涎腺高肺转移性腺样囊性癌 (ACC M)细胞的影响。方法 采用细胞形态学观察及PCR TRAP端粒酶活性检测法来观察其形态学和端粒酶活性的变化。结果 反义寡核苷酸在转染ACC M细胞后 72小时内 ,其形态学改变不明显 ,其端粒酶活性明显受到抑制 (P <0 .0 1)。结论 AS ODN可显著抑制ACC M的端粒酶活性 ,提示AS ODN可抑制ACC M细胞的生长 ,抑制效果可能与AS ODN的剂量、转染效率等因素有关。

Objective To study the effect of telomerase antisense oligodeoxy-ribonucleo-tide (AS-ODN)on human adenoid cystic carcinoma which can metastasize highly to lungs(ACC-M).Methods To observe the change of the morphology and telomerase activity with microscopy and telomeric repeat amplification protocol (TRAP).Results During 24～72 hours after AS-ODN transformation,the telomerase activity of ACC-M cells was inhibited obviously(p<0.01),but has little change on morphology.Conclusion In vitro,the telomerase activity of ACC-M cell line can be inhibited by AS-ODN, to indicate that the growth of ACC-M cell can be inhibited by AS-ODN,and the inhibition effect was influenced by the dosage of AS-ODN and other factors.