脑梗死患者纤维蛋白原β-148C/T基因多态性的研究

The study on the polymorphism of beta fibrinogen gene-148C/T in the patients with cerebral infarction

目的 探讨脑梗死 (CI)患者纤维蛋白原 (Fg)Bβ基因启动区多态性位点 β 14 8C/T的基因多态性频率分布及其与Fg功能和水平的关系。方法 用聚合酶反应 限制性片段长度多态性 (RFLP)分析方法 ,对CI组 (15 1例 )和健康对照组 (113人 )进行研究 ,应用Fg功能自动化检测系统检测血浆Fg分子功能参数 ,用比浊法测Fg水平。结果 CI组与对照组T等位基因的频率分别为 0 .32 1和 0 .2 4 8,两组比较有显著性差异 (P <0 0 5 ) ;CI组和对照组Fg分子功能 (FMPV/ODmax)分别是 (4 .4 73± 0 .5 0 9)和 (3.716± 0 .4 35 ) ,两组比较有极显著差异 (P <0 .0 1) ,Fg水平 (S)分别为 (3.90± 0 .37)和 (2 .87± 0 .5 1) ,两组比较有极显著差异 (P <0 .0 1)。Fgβ 14 8C/T基因多态性与Fg水平 (r=0 .5 2 ,P <0 .0 0 1)和Fg分子功能 (r=0 .4 5 ,P <0 .0 0 2 )间存在正相关。结论 Fgβ 14 8C/T基因多态性与血浆Fg分子功能和Fg水平具有相关性 ,Fg分子功能增强和Fg水平升高是CI的危险因素 ,Fgβ 14 8C/T基因多态性与CI易患性相关联。

Objective To investigate the frequency of beta fribrinogen(Fg) gene 148C/T polymorphism and its association with the function and level of plasma fibrinogen in patients with cerebral infarction(CI).Methods The beta Fg gene 148C/T polymorphisms of 151 patients with CI and 113 health people were analyzed by restriction fragment length polymorphism(RFLP).Fg molecular reactivities were measured by the automatic detective system and Fg levels were determined by turbidimetry.Results The frequencies of T allele in CI patients and healthy controls were 0.321 and 0.248 respectively( P <0.05).The plasma Fg molecular reactivities were enhanced in cases of CI than those in healthy controls, 4.473±0.509 and 3.716±0.435( P <0.01) respectively.The plasma Fg levels of CI patients were significantly higher than those of healthy controls, 3.90±0.37 and 2.87±0.51( P <0.01),respectively. The beta Fg gene 148C/T polymorphisms was significantly associated with plasma fibrinogen levels( r =0.52, P <0.001)and Fg molecular reactivity ( r =0.45, P <0.002).Conclusions The beta Fg gene 148C/T polymorphism is correlated to the molecular reactivity and plasma of Fg level.The increased plasma Fg level and enhanced Fg molecular reactivity are both risk factors of CI.CI susceptibility is associated with beta Fg gene 148C/T polymorphism.