氟对成骨细胞增殖分化的影响及维生素C的拮抗作用

Effects of fluoride on proliferation and differentiation of rat osteoblasts in vitro and the antagonistic action of vitamin C

目的 研究不同剂量的氟对体外培养大鼠乳鼠颅骨成骨细胞增殖分化的影响及维生素C的拮抗作用。方法 酶消化法分离成骨细胞 ;AlamarBlue摄入法检测细胞增殖 ;ELISA方法测定碱性磷酸酶 (ALP)活力。结果 氟化钠浓度 0 .10～ 1.0 0mmol L刺激细胞增殖 ,细胞活力明显增强 ,与对照组相比差异有显著性 (P <0 0 5 ) ;2 .0 0mmol L以上细胞活力下降 ,抑制细胞增殖 ;0 .0 1～ 0 .0 5mmol L增强细胞ALP活力 ,0 .10mmol L以上降低细胞ALP活力 ,与对照组相比 ,差异均有显著性 (P <0 .0 5 )。加入维生素C ,2mmol L氟化钠仍能促使细胞增殖分化。结论 氟对成骨细胞的增殖与分化具有双向作用 ;维生素C能拮抗较高浓度氟化钠对成骨细胞增殖分化的抑制。

Objective To study the effects of fluoride on the proliferation and differentiation of osteoblasts in sucking rats and the antagonism of vitamin C in vitro. Methods The enzyme digesting method was used to isolate the rat osteoblasts;the proliferative response was determined by the percents of reduced alamarBlue;the activity of alkaline phosphatase(ALP) was measured by ELISA method. Results The proliferation of sucking rat osteoblasts was increased at 0.10～1.00 mmol/L of NaF,whereas inhibited at ≥2.00 mmol/L.ALP activity was increased at 0.01～0.05 mmol/L of NaF,and decreased at ≥0.10 mmol/L.The inhibition on proliferation and differentiation at 2 mmol/L NaF was antagonized by vitamin C. Conclusion Fluoride had a two-phase effect on osteoblasts,vitamin C could antagonize the inhibitory effect of higher concentration of fluoride on proliferation and differentiation of osteoblasts.