RT-PCR法测定牵拉性撕脱伤血管上P-选择素量的变化

Change of expression of P-selectin in avulsion-injured vessels

目的 探讨牵拉性撕脱伤血管上的P 选择素的变化。方法 设定 6 0、70、80及 90g的牵拉力 ,利用血管损伤模型造成不同的血管损伤 ,用RT PCR法测定损伤血管上P 选择素量的变化。结果 撕脱伤血管上P 选择素mRNA表达随牵拉力增加而升高 ,静脉的表达要高于动脉。结论 结合以前的相关实验 ,提示P 选择素与血栓形成关系密切。

Objective To determine the change of P-selectin in avulsion-injured vessels. Methods Different stretch forces of 60, 70, 80 and 90?g were applied to a vascular injury model.The expression changes of P-selectin were evaluated by RT-PCR. Results The expression of P-selectin mRNA in the injured vessels increased with the stretch force. Conclusion The result associated with our previous study indicated that P-selectin may be involved in thrombosis.;