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Effect of Estrogen on Telomerase Activity in Human Breast Cancer Cells

Effect of Estrogen on Telomerase Activity in Human Breast Cancer Cells
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摘要 To investigate the effects of estrogen(E 2) on telomerase activity and its mechanism in human breast cancer cells, estrogen receptor positive MCF-7 cells were treated with different concentrations of E 2 Telomerase activity was measured by using TRAP-ELISA method, the cell cycle phases analyzed by using flow cytometry, and the expression of Cyclin D 1 detected by using immunohistochemistry method The results showed that telomerase activity levels were increased in MCF-7 cells treated with 10 -8 mol/L E 2 during the observed period ( P <0 05), and E 2 increased telomerase activity levels in a dose-dependent manner(10 -10 -10 -8 mol/L); Simultaneously, the cell cycle phases of MCF-7 cells treated with 10 -8 mol/L E 2 were changed significantly: G 0/G 1 phase decreased from 60 52% to 50 93%, S phase increased from 29 03% to 30 83%; However, the expression of Cyclin D 1 was decreased It was concluded that estrogen can upregulate telomerase activity of MCF-7 cells, and the effect can be blocked by antiestrogen tamoxifen Its mechanism may be closely associated with modulation of cell cycle phases To investigate the effects of estrogen(E 2) on telomerase activity and its mechanism in human breast cancer cells, estrogen receptor positive MCF-7 cells were treated with different concentrations of E 2 Telomerase activity was measured by using TRAP-ELISA method, the cell cycle phases analyzed by using flow cytometry, and the expression of Cyclin D 1 detected by using immunohistochemistry method The results showed that telomerase activity levels were increased in MCF-7 cells treated with 10 -8 mol/L E 2 during the observed period ( P <0 05), and E 2 increased telomerase activity levels in a dose-dependent manner(10 -10 -10 -8 mol/L); Simultaneously, the cell cycle phases of MCF-7 cells treated with 10 -8 mol/L E 2 were changed significantly: G 0/G 1 phase decreased from 60 52% to 50 93%, S phase increased from 29 03% to 30 83%; However, the expression of Cyclin D 1 was decreased It was concluded that estrogen can upregulate telomerase activity of MCF-7 cells, and the effect can be blocked by antiestrogen tamoxifen Its mechanism may be closely associated with modulation of cell cycle phases
出处 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2003年第3期286-287,293,共3页 华中科技大学学报(医学英德文版)
关键词 breast cancer cells TELOMERASE ESTROGEN Cyclin D 1 breast cancer cells telomerase estrogen Cyclin D 1
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