白藜三醇对黄嘌呤/黄嘌呤氧化酶所致平滑机细胞增殖的干预作用

Intervention of Resveatrol in Proliferation of Cultured Rabbit Aortic Smooth Muscle Cells In-duced by Xanthine and Xanthine Oxidase

目的探讨红葡萄酒 (RW)是否对血管SMC增殖具有抑制作用。方法以培养幼兔主动脉平滑肌细胞为研究对象 ,分别给予不同浓度黄嘌呤/黄嘌呤氧化酶和/或白藜三醇 ,采用免疫组织化学、3H -TdR掺入试验和MTT法技术检测不同处理对平滑肌细胞的增殖作用。结果RES呈剂量依赖性拮抗X/XO对VSMC的促增生效应 ;终浓度50umol/L的RES对X200umol/L/XO200u/L所致促VSMC的增殖效应即有抑制作用(P<0.05) ;终浓度200umol/L的RES对X200umol/L/XO200u/L所致促VSMC的增殖效应则具有极显著的抑制作用 (P<0.001)。结论红葡萄酒中的有效成份白藜三醇可拮抗黄嘌呤/黄嘌呤氧化酶所致平滑肌细胞的作用 ,这可能有助于减轻或防治氧化损伤对血管平滑肌细胞增殖的影响。

Objective To investigate the role of resveatrol on the proliferation in cultured rabbit aortic smooth muscle cells(SMC)induced by xanthine and xanthine oxidase.Methods Effects of prolifera-tion in cultured rabbit aortic smooth muscle cells(SMC)were detected by immunohistochemitry, 3 H-Thymi-dine( 3 H-TdR)incorporation and3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazoliumbromide metabolism(MTT).Results Resveatrol(RES),an effective composition in red wine was confirmed to inhibit3H-Thymidine( 3 H-TdR)incorporation,3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazoliumbromide metabolism(MTT)and PCNA expression in rabbit aortic SMC which were promoted by the oxygen free radicals in-duced by xanthine and xanthine oxidase.Conclusions Resveatrol may inhibit xanthine and xanthine oxidase induced proliferation in cultured rabbit aortic smooth muscle cells(SMC),which might play an important role in preventing active oxygen species lesion to atherosclerosis.